Genome-Wide Identification of MicroRNAs in Response to Cadmium Stress in Oilseed Rape (Brassica napus L.) Using High-Throughput Sequencing

International Journal of Molecular Sciences
Hongju JianLiezhao Liu

Abstract

MicroRNAs (miRNAs) have important roles in regulating stress-response genes in plants. However, identification of miRNAs and the corresponding target genes that are induced in response to cadmium (Cd) stress in Brassica napus remains limited. In the current study, we sequenced three small-RNA libraries from B. napus after 0 days, 1 days, and 3 days of Cd treatment. In total, 44 known miRNAs (belonging to 27 families) and 103 novel miRNAs were identified. A comprehensive analysis of miRNA expression profiles found 39 differentially expressed miRNAs between control and Cd-treated plants; 13 differentially expressed miRNAs were confirmed by qRT-PCR. Characterization of the corresponding target genes indicated functions in processes including transcription factor regulation, biotic stress response, ion homeostasis, and secondary metabolism. Furthermore, we propose a hypothetical model of the Cd-response mechanism in B. napus. Combined with qRT-PCR confirmation, our data suggested that miRNAs were involved in the regulations of TFs, biotic stress defense, ion homeostasis and secondary metabolism synthesis to respond Cd stress in B. napus.

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Citations

Mar 27, 2019·International Journal of Molecular Sciences·Baoxiang WangMingpu Tan
Mar 13, 2020·Frontiers in Plant Science·Neeta LohaniPrem L Bhalla
Feb 13, 2021·Biomolecules·Saquib WaheedZhangli Hu
Jul 11, 2021·Plant Physiology and Biochemistry : PPB·Sudhakar Srivastava, Penna Suprasanna

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Datasets Mentioned

BETA
SRP118762

Methods Mentioned

BETA
Illumina sequencing
transgenic

Software Mentioned

Primer Premier
Blast2GO
IDGE6
SOAP2

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