Genome Wide Identification of Mutational Hotspots in the Apicomplexan Parasite Neospora caninum and the Implications for Virulence

Genome Biology and Evolution
Larissa CalarcoJ Ellis

Abstract

Neospora caninum is an apicomplexan parasite responsible for neosporosis, a disease causing hind limb paralysis in dogs and abortion in cattle, resulting in substantial economic losses to beef and dairy industries. Marked differences in pathogenicity exist between N. caninum strains suggesting that intrinsic genetic differences exist between them. These differences likely exist in genes expressed during the tachyzoite lifecycle stage which is responsible for the pathogenesis of neosporosis. An improved understanding of these genetic differences is essential to understanding N. caninum virulence, though such knowledge is scarce. Using a variant detection workflow we compared the tachyzoite transcriptomes of two N. caninum strains with different virulence properties: NC-Liverpool (virulent) and NC-Nowra (avirulent). This workflow identified 3130 SNPs and 6123 indels between the strains, and nine markers capturing 30 variants were Sanger sequenced for both strains. Sequencing of these loci was extended to an additional eight strains and subsequent phylogenetic analysis supported a genetic population structure comprised of two major clades with no geographical segregation. Sequence polymorphisms within coding regions of tachyzoite-...Continue Reading

References

May 1, 1991·Trends in Biochemical Sciences·M Goebl, M Yanagida
Apr 1, 1994·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·B C BarrP A Conrad
Nov 20, 1993·The Veterinary Record·J BarberB Tennant
Dec 2, 1996·Veterinary Parasitology·J P Dubey, D S Lindsay
Jun 29, 1999·Veterinary Parasitology·D S LindsayR B Duncan
Aug 24, 1999·Veterinary Parasitology·J P Dubey
Oct 6, 1999·Genome Research·X Huang, A Madan
Mar 16, 2000·Molecular and Biochemical Parasitology·J L LovettL D Sibley
Feb 17, 2001·Current Opinion in Structural Biology·J H LaityP E Wright
Jul 27, 2001·Parasitology·A SchockJ M Wastling
Oct 12, 2001·International Journal for Parasitology·E A InnesD Buxton
Dec 6, 2002·Australian Veterinary Journal·C M D MillerJ T Ellis
Apr 2, 2003·The Korean Journal of Parasitology·J P Dubey, John Dubey
Apr 30, 2003·The Journal of Infectious Diseases·Jiang-Ti KongJohn C Boothroyd
Aug 19, 2003·Journal of Comparative Pathology·S W MaleyE A Innes
Apr 28, 2004·Journal of Molecular Biology·Lukas KällErik L L Sonnhammer
Feb 3, 2005·The Journal of Experimental Medicine·Odile CérèdeMaryse Lebrun
Jun 28, 2005·Nucleic Acids Research·E QuevillonR Lopez
Jul 21, 2005·New Zealand Veterinary Journal·M P Reichel, J T Ellis
May 23, 2006·Journal of Comparative Pathology·J P DubeyW Wouda
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jon P BoyleJohn C Boothroyd
Jul 11, 2006·Annual Review of Genomics and Human Genetics·Pauline C Ng, Steven Henikoff
Aug 4, 2006·The Journal of Parasitology·Javier Regidor-CerrilloLuis M Ortega-Mora
Aug 29, 2006·PLoS Pathogens·My-Hang Huynh, Vern B Carruthers
Jun 6, 2007·The Journal of Small Animal Practice·M P ReichelJ P Dubey
Nov 8, 2008·PLoS Computational Biology·Sheila M ReynoldsWilliam Stafford Noble
Nov 8, 2008·Bioinformatics·Tim CarverJulian Parkhill
Jun 6, 2009·Clinical and Vaccine Immunology : CVI·Susana SousaMarie-Laure Dardé
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jun 23, 2009·Genome Research·Martin KrzywinskiMarco A Marra
Aug 18, 2009·Molecular and Cellular Probes·Sarwat Al-QassabJohn Ellis
Jan 14, 2010·Current Protocols in Molecular Biology·Daniel BlankenbergJames Taylor
Jun 22, 2010·Bioinformatics·Daniel BlankenbergUNKNOWN Galaxy Team
Jan 12, 2011·Nature Biotechnology·James T RobinsonJill P Mesirov
Feb 16, 2011·International Journal for Parasitology·Asis KhanL David Sibley
Mar 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michael L ReeseJon P Boyle

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Citations

Jun 13, 2019·BMC Veterinary Research·Darwin ChangoluisaUNKNOWN working group “Applied Microbiology” of the School of Biological Sciences and Engineering at Yachay Tech University
Feb 20, 2020·Parasites & Vectors·Bingxin ZhouJing Liu
Jun 27, 2020·Pathogens·Michael P ReichelJohn T Ellis
Oct 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Asis KhanMichael E Grigg
Dec 21, 2019·International Journal for Parasitology·Larissa CalarcoJohn Ellis

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Methods Mentioned

BETA
PCR
genotyping
RNA-seq
PCRs
electrophoresis
GTPase
multilocus sequencing
protein folding

Software Mentioned

EST
CD
BLASTX
BLASTN
R package
InterProScan
Genome Analysis Toolkit ( GATK )
Integrative Genomics Viewer ( IGV )
HIT
TopHat

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