Genome-wide Identification of PP2C Genes and Their Expression Profiling in Response to Drought and Cold Stresses in Medicago truncatula

Scientific Reports
Qi YangRuigang Wang

Abstract

Type 2 C protein phosphatases (PP2Cs) represent the major group of protein phosphatases in plants and play important roles in various plant processes. In this study, 94 MtPP2C genes were identified from Medicago truncatula and further phylogenetically classified into 13 subfamilies, as supported by exon-intron organization and conserved motif composition. Collinearity analysis indicated that segmental duplication events played a crucial role in the expansion of MtPP2C gene families in M. truncatula. Furthermore, the expression profiles of MtPP2Cs under different abiotic treatments were analyzed using qRT-PCR. Results showed that these MtPP2Cs genes displayed different expression patterns in response to drought, cold and ABA stress conditions and some of the key stress responsive MtPP2Cs genes have been identified. Our study presents a comprehensive overview of the PP2C gene family in M. truncatula, which will be useful for further functional characterization of MtPP2Cs in plant drought and cold stress responses.

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Citations

Jun 21, 2019·International Journal of Molecular Sciences·Jitendra KumarShiv Kumar
Mar 23, 2019·International Journal of Molecular Sciences·Hamna ShazadeeXinyu Wang
Jul 29, 2020·International Journal of Molecular Sciences·Kalaivani K Nadarajah

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Batch CD - search
WoLF PSORT
MapInspect
PlantCARE
ExPASy
MEGA6
MEGA
Graphpad prism
Pfam
Compute pI / MW

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