Genome-Wide Identification of Sorghum bicolor Laccases Reveals Potential Targets for Lignin Modification

Frontiers in Plant Science
Jinhui WangYinxin Li

Abstract

Laccase is a key enzyme in plant lignin biosynthesis as it catalyzes the final step of monolignols polymerization. Sweet sorghum [Sorghum bicolor (L.) Moench] is considered as an ideal feedstock for ethanol production, but lignin greatly limits the production efficiency. No comprehensive analysis on laccase has ever been conducted in S. bicolor, although it appears as the most promising target for engineering lignocellulosic feedstock. The aim of our work is to systematically characterize S. bicolor laccase gene family and to identify the lignin-specific candidates. A total of twenty-seven laccase candidates (SbLAC1-SbLAC27) were identified in S. bicolor. All SbLACs comprised the equivalent L1-L4 signature sequences and three typical Cu-oxidase domains, but exhibited diverse intron-exon patterns and relatively low sequence identity. They were divided into six groups by phylogenetic clustering, revealing potential distinct functions, while SbLAC5 was considered as the closest lignin-specific candidate. qRT-PCR analysis deciphered that SbLAC genes were expressed preferentially in roots and young internodes of sweet sorghum, and SbLAC5 showed high expression, adding the evidence that SbLAC5 was bona fide involved in lignin biosynt...Continue Reading

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Citations

Feb 9, 2018·International Journal of Molecular Sciences·Waqar IslamLiande Wang
May 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoke PingLiezhao Liu
Oct 30, 2020·International Journal of Molecular Sciences·Duc Quan NguyenChristopher P L Grof
Feb 27, 2021·Journal of Integrative Plant Biology·Xuehai ZhengTing Xue

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Methods Mentioned

BETA
glycosylation
environmental stresses
fluorescence microscopy
transgenic

Software Mentioned

SMART
BLASTP
psRNATarget
NetNGlyc
Genestudio
NetPhos
TargetP
MEGA
SignalP

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