Genome-wide identification of transcription factors that are critical to non-small cell lung cancer

Cancer Letters
Da-Lin ZhangGuang-Biao Zhou

Abstract

To systematically unveil transcription factors (TFs) that are critical to lung carcinogenesis, here we conducted a genome-wide lethality screening in non-small cell lung cancer (NSCLC) cells and reported that among the 1530 TFs tested, 21 genes were required for NSCLC cell proliferation and were negatively or positively associated with overall survival (OS) of patients with NSCLC. These included 11 potential tumor suppressing genes (AFF3, AhR, AR, CBFA2T3, CHD4, KANK2, NR3C2, PTEN, PRDM16, RB1, and STK11) and 10 potential oncogenic TFs (BARX1, DLX6, ELF3, EN1, ETV1, FOXE1, HOXB7, IRX4, IRX5, and SALL1). The expression levels of IRX5 were positively associated with OS of smoker and inversely associated with OS of non-smoker patients with lung adenocarcinoma. We showed that tobacco carcinogen benzo(a)pyrene (BaP) induced upregulation of IRX5 in lung epithelial cells, and Cyclin D1 was a downstream target of IRX5. Furthermore, silencing of IRX5 by lentivirus mediated transfection of short hairpin RNA significantly inhibited tumor growth in nude mice. These results indicate that tobacco smoke can modulate TFs to facilitate lung carcinogenesis, and inhibition of IRX5 may have therapeutic potentials in NSCLCs.

References

Jan 1, 1989·Cytogenetics and Cell Genetics·Y S FanT B Shows
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·A SimeoneK Huebner
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Apr 6, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·R H CostaL Lim
Jul 9, 2002·Current Opinion in Genetics & Development·José Luis Gómez-Skarmeta, Juan Modolell
Apr 18, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Christopher TheokliRichard Morgan
Jul 31, 2004·Science·Raffaella SordellaJeffrey Settleman
Oct 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aphrodite BilioniHelen McNeill
Nov 22, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Minke van TuylMartin Post
Dec 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Eric B HauraGerold Bepler
Feb 9, 2006·Nature Biotechnology·Nathalie MaloRobert Nadon
Jun 16, 2007·Genome Biology·Allen DayStanley F Nelson
Aug 7, 2007·Nature·Hongbin JiKwok-Kin Wong
Nov 13, 2007·Biochimica Et Biophysica Acta·Yun ZhuRyoiti Kiyama
Dec 7, 2007·The Journal of Clinical Investigation·Sizhi Paul GaoJacqueline F Bromberg
Jun 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anne MyrthueTomasz M Beer
Sep 26, 2008·The New England Journal of Medicine·Roy S HerbstScott M Lippman
Dec 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michal MarzecMariusz A Wasik
Jul 25, 2009·Nature Reviews. Cancer·Jeffrey A Engelman
Aug 19, 2010·Epigenetics : Official Journal of the DNA Methylation Society·Yan JiaMingzhou Guo
Jan 27, 2011·European Journal of Immunology·Sabine J WölfleKlaus Heeg
Jan 5, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Kristin BergethonA John Iafrate
May 15, 2012·Nature Genetics·Carine BonnardBruno Reversade
Aug 23, 2012·Molecular Carcinogenesis·Weiwei YuanShiyong Wu
Sep 5, 2012·International Journal of Cancer. Journal International Du Cancer·Stephen S Hecht
Sep 11, 2012·Nature·UNKNOWN Cancer Genome Atlas Research Network
Sep 12, 2013·European Journal of Human Genetics : EJHG·Xing XuRobert J Klein
Aug 1, 2014·Nature·UNKNOWN Cancer Genome Atlas Research Network

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Citations

Mar 13, 2020·Journal of Cellular Biochemistry·Xun SunTing Wang
Oct 2, 2019·Frontiers of Medicine·Yanfei ZhangGuangbiao Zhou
Apr 16, 2020·International Journal of Molecular Sciences·Amelia CasamassimiCiro Abbondanza
Dec 12, 2020·Frontiers in Genetics·Ning LanWenzhen Yuan
Jun 2, 2021·Journal of Cellular and Molecular Medicine·Ping YuYing Chen
Sep 21, 2021·Frontiers in Cell and Developmental Biology·Haihui ZhongYefeng Shen

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