Genome-Wide Identification Reveals That Nicotiana benthamiana Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus.

Frontiers in Plant Science
Xinyang WuHongying Zheng

Abstract

Hypersensitive response (HR)-like cell death is an important mechanism that mediates the plant response to pathogens. In our previous study, we reported that NbHIR3s regulate HR-like cell death and basal immunity. However, the host genes involved in HR have rarely been studied. Here, we used transcriptome sequencing to identify Niben101Scf02063g02012.1, an HR-like lesion inducing protein (HRLI) in Nicotiana benthamiana that was significantly reduced by turnip mosaic virus (TuMV). HRLIs are uncharacterized proteins which may regulate the HR process. We identified all six HRLIs in N. benthamiana and functionally analyzed Niben101Scf02063g02012.1, named NbHRLI4, in response to TuMV. Silencing of NbHRLI4 increased TuMV accumulation, while overexpression of NbHRLI4 conferred resistance to TuMV. Transient overexpression of NbHRLI4 caused cell death with an increase in the expression of salicylic acid (SA) pathway genes but led to less cell death level and weaker immunity in plants expressing NahG. Thus, we have characterized NbHRLI4 as an inducer of cell death and an antiviral regulator of TuMV infection in a SA-mediated manner.

References

Apr 12, 2000·The Plant Journal : for Cell and Molecular Biology·H G Kang, K B Singh
Feb 24, 2001·Plant Molecular Biology·M C Heath
Oct 20, 2001·Current Opinion in Plant Biology·E P Beers, J M McDowell
Sep 11, 2002·The Plant Journal : for Cell and Molecular Biology·Yule LiuS P Dinesh-Kumar
Feb 7, 2004·Cellular Microbiology·Jean T Greenberg, Nan Yao
Apr 9, 2004·Nature Reviews. Molecular Cell Biology·Eric Lam
Apr 30, 2009·Annual Review of Phytopathology·A Corina VlotDaniel F Klessig
May 22, 2009·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Mar 3, 2010·Molecular Plant-microbe Interactions : MPMI·Gerardo SánchezMaría Rosa Marano
Aug 11, 2012·Molecular Plant-microbe Interactions : MPMI·Aureliano BombarelyGregory B Martin
Oct 17, 2012·The Plant Journal : for Cell and Molecular Biology·Jae Sung ShimYang Do Choi
Sep 16, 2014·Molecular Plant Pathology·Mazen Alazem, Na-Sheng Lin
Nov 22, 2014·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Dec 15, 2015·Current Opinion in Virology·Tamara D Collum, James N Culver
Dec 3, 2016·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Jul 21, 2017·Journal of Virology·Eun Jin JeonKenji S Nakahara
Aug 28, 2017·Current Opinion in Virology·Peter PalukaitisJohn P Carr
Aug 31, 2018·Plant Physiology·Hui-Yang XuJian-Zhong Liu
Jan 4, 2019·The Plant Journal : for Cell and Molecular Biology·Yuhuan MiaoLongfu Zhu
Sep 13, 2019·Plant Physiology and Biochemistry : PPB·Yi-He YuDa-Long Guo

❮ Previous
Next ❯

Software Mentioned

MEGA7
Image J
MEME
DNAMAN
TBtools
NbHRLIs
PlantRegMap
Blast
PlantCARE

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.