Mar 29, 2016

Genome-wide measurement of spatial expression in patterning mutants of Drosophila melanogaster

BioRxiv : the Preprint Server for Biology
Peter A Combs, Michael B Eisen

Abstract

Genome sequencing has become commonplace, but the understanding of how those genomes ultimately specify cell fate during development is still elusive. Extrapolating insights from deep investigation of a handful of developmentally important Drosophila genes to understanding the regulation of all genes is a major challenge. The developing embryo provides a unique opportunity to study the role of gene expression in pattern specification; the precise and consistent spatial positioning of key transcription factors essentially provides separate transcriptional-readout experiments at a critical point in development. We cryosectioned and sequenced mRNA from single Drosophila melanogaster embryos at the blastoderm stage to screen for spatially-varying regulation of transcription. Expanding on our previous screening of wild type embryos, here we present data from dosage mutants for key maternally provided regulators, including depletion of zelda and hunchback and both over-expression and depletion of bicoid. These data recapitulate all of the expected patterning changes driven by these regulators; for instance, we show spatially-confined up-regulation of expression in the bicoid over-expression condition, and down-regulation of those gen...Continue Reading

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Mentioned in this Paper

Embryo
Genome-Wide Association Study
Congenital Kyphosis
hb
Patterns
Cell Fate
Gene Knockdown Techniques
Genome
Genes
Regulation of Biological Process

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