Genome-wide methylation profiling and a multiplex construction for the identification of body fluids using epigenetic markers

Forensic Science International. Genetics
Hwan Young LeeKyoung-Jin Shin

Abstract

The identification of body fluids found at crime scenes can contribute to solving crimes by providing important insights into crime scene reconstruction. In the present study, body fluid-specific epigenetic marker candidates were identified from genome-wide DNA methylation profiling of 42 body fluid samples including blood, saliva, semen, vaginal fluid and menstrual blood using the Illumina Infinium HumanMethylation450 BeadChip array. A total of 64 CpG sites were selected as body fluid-specific marker candidates by having more than 20% discrepancy in DNA methylation status between a certain type of body fluid and other types of body fluids and to have methylation or unmethylation pattern only in a particular type of body fluid. From further locus-specific methylation analysis in additional samples, 1 to 3 CpG sites were selected for each body fluid. Then, a multiplex methylation SNaPshot reaction was constructed to analyze methylation status of 8 body fluid-specific CpG sites. The developed multiplex reaction positively identifies blood, saliva, semen and the body fluid which originates from female reproductive organ in one reaction, and produces successful DNA methylation profiles in aged or mixed samples. Although it remains ...Continue Reading

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Citations

Jun 10, 2015·Forensic Science International. Genetics·Hwan Young LeeKyoung-Jin Shin
Mar 15, 2016·Forensic Science, Medicine, and Pathology·Yu-Chih LinHsing-Mei Hsieh
Mar 30, 2016·Forensic Science International : Synergy·E De VittoriV Romano Spica
Oct 2, 2015·International Journal of Epidemiology·Caroline L ReltonGeorge Davey Smith
Oct 23, 2016·Forensic Science International. Genetics·Yu-Chih LinHsing-Mei Hsieh
May 12, 2018·Electrophoresis·Rebecca RichardsSallyAnn Harbison
Jan 27, 2018·International Journal of Legal Medicine·Hannah HoltkötterMarielle Vennemann
Nov 13, 2019·International Journal of Legal Medicine·Farzeen KaderAdemola O Olaniran
Apr 22, 2016·BMB Reports·Hwan Young LeeKyoung-Jin Shin
Nov 13, 2019·Cellular and Molecular Life Sciences : CMLS·Jennifer SteensDiana Klein
Nov 5, 2016·Electrophoresis·Sang-Eun JungHwan Young Lee
Dec 31, 2020·Diagnostics·Anna RocchiIsabella Spinetti
Nov 22, 2020·Forensic Science International. Genetics·Andrea Patrizia SalzmannCordula Haas
Nov 28, 2017·Molecular and Cellular Probes·Beccy Stafford-AllenSimon Wells
Apr 2, 2021·Emerging Topics in Life Sciences·Penelope R Haddrill
Apr 10, 2021·Legal Medicine·Chong Min ChoungSi-Keun Lim
Jun 27, 2021·Science & Justice : Journal of the Forensic Science Society·Masanori DoiMigiwa Asano
Oct 13, 2021·International Journal of Legal Medicine·Helen KonradMicaela Poetsch

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