Genome-Wide Profiling of Long Noncoding RNA Expression Patterns in Women With Repeated Implantation Failure by RNA Sequencing

Reproductive Sciences
Min-Yan ChenShang-Wei Li

Abstract

To compare long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression levels in endometrium between patients with repeated implantation failure (RIF) following in vitro fertilization (IVF)-embryo transfer and control women. RNA sequencing (RNA-seq) and alignments were performed to identify lncRNAs and mRNAs using endometrial samples collected from 3 patients and 3 control women. A subset of 10 differentially expressed lncRNAs and 6 mRNAs were validated in all participants using quantitative reverse transcription polymerase chain reaction. The potential biological roles of identified lncRNAs were predicted via coexpressed mRNA annotations. Twenty patients with RIF and 30 control women were recruited for validation. We identified 1202 differentially expressed genes, including 742 lncRNAs and 460 mRNAs, in mid-secretory phase endometrial tissue from patients with RIF following IVF compared to control women. We analyzed the target genes of the lncRNAs and uncovered 148 lncRNAs corresponding to 147 cis-regulated target genes. The cis-regulated target genes of these significantly differentially expressed lncRNAs were clustered into several pathways, such as the tumor necrosis factor signaling pathway, the Toll-like receptor sign...Continue Reading

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Citations

Oct 17, 2018·Reproductive Biology and Endocrinology : RB&E·Xueying ZhangManhua Cui
Dec 10, 2019·Cellular and Molecular Life Sciences : CMLS·Hong LiuAihua Liao
Oct 23, 2019·Biochimica Et Biophysica Acta. Reviews on Cancer·Subash C GuptaKishore B Challagundla
May 27, 2021·Clinical Epigenetics·Alejandra Monserrat Retis-ResendizEdgar Ricardo Vázquez-Martínez
Jun 26, 2021·Reproductive Biomedicine Online·Piotr PierzyńskiChristophe Blockeel

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