Genome-wide profiling of PRC1 and PRC2 Polycomb chromatin binding in Drosophila melanogaster

Nature Genetics
Bas TolhuisMaarten van Lohuizen

Abstract

Polycomb group (PcG) proteins maintain transcriptional repression of developmentally important genes and have been implicated in cell proliferation and stem cell self-renewal. We used a genome-wide approach to map binding patterns of PcG proteins (Pc, esc and Sce) in Drosophila melanogaster Kc cells. We found that Pc associates with large genomic regions of up to approximately 150 kb in size, hereafter referred to as 'Pc domains'. Sce and esc accompany Pc in most of these domains. PcG-bound chromatin is trimethylated at histone H3 Lys27 and is generally transcriptionally silent. Furthermore, PcG proteins preferentially bind to developmental genes. Many of these encode transcriptional regulators and key components of signal transduction pathways, including Wingless, Hedgehog, Notch and Delta. We also identify several new putative functions of PcG proteins, such as in steroid hormone biosynthesis. These results highlight the extensive involvement of PcG proteins in the coordination of development through the formation of large repressive chromatin domains.

References

Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·S HenikoffB van Steensel
Jan 4, 2001·Cell Death and Differentiation·E H Baehrecke
Mar 10, 2001·Nature Genetics·B van SteenselS Henikoff
Mar 13, 2002·The EMBO Journal·Luc E G RietveldHendrik G Stunnenberg
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·James T WarrenLawrence I Gilbert
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bas van SteenselHarmen J Bussemaker
Jul 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ling V SunKevin P White
Dec 19, 2003·Nucleic Acids Research·Todd W HarrisLincoln D Stein
Aug 19, 2004·Cell·Merel E Valk-LingbeekMaarten van Lohuizen
Sep 28, 2004·EMBO Reports·Achim BreilingValerio Orlando
Dec 1, 2004·Annual Review of Genetics·Leonie Ringrose, Renato Paro
Mar 26, 2005·Nature·Jérôme DéjardinGiacomo Cavalli
Aug 5, 2005·Development·Gabriella FiczDonna J Arndt-Jovin
Nov 30, 2005·Molecular and Cellular Biology·Inmaculada Hernández-MuñozMaarten van Lohuizen
Apr 15, 2006·PLoS Biology·Nicolas NègreGiacomo Cavalli

❮ Previous
Next ❯

Citations

Oct 15, 2008·Chromosoma·Elzo de Wit, Bas van Steensel
Mar 20, 2008·Molecular Genetics and Genomics : MGG·Gabriella KozmaLászló Sipos
Sep 2, 2011·Cell Death & Disease·H RichlyL Di Croce
Jan 21, 2011·Nature·Raphaël Margueron, Danny Reinberg
Sep 15, 2009·Nature Genetics·Anne-Kathrin ClassenDavid Bilder
Jun 5, 2007·Nature Protocols·Maartje J VogelBas van Steensel
Oct 25, 2006·Nature Reviews. Cancer·Anke Sparmann, Maarten van Lohuizen
Aug 22, 2006·Nature Reviews. Molecular Cell Biology·Jesús Gil, Gordon Peters
Sep 10, 2009·Nature Reviews. Molecular Cell Biology·Jeffrey A Simon, Robert E Kingston
Jun 15, 2010·Nature Structural & Molecular Biology·Selma El Messaoudi-AubertGordon Peters
Oct 8, 2013·Nature Structural & Molecular Biology·Luciano Di Croce, Kristian Helin
Jun 15, 2010·Oncogene·E C ToomeyS L Lessnick
Jan 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Dusan ZencakYvan Arsenijevic
Sep 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Margaret E McLaughlin-DrubinKarl Munger
Feb 2, 2011·Molecular & Cellular Proteomics : MCP·Julien VandammePierre-Olivier Angrand
May 23, 2013·Omics : a Journal of Integrative Biology·Shahram GolbabapourA Hamid A Hadi
Mar 2, 2012·Human Molecular Genetics·Alireza Baradaran-HeraviCornelius F Boerkoel
Feb 12, 2011·Nucleic Acids Research·Sílvia Pérez-LluchMontserrat Corominas
Jan 21, 2011·Nucleic Acids Research·Nickolai A TchurikovYuri V Kravatsky
Mar 15, 2012·Nucleic Acids Research·Jia ZengJianpeng Ma
Mar 24, 2010·Current Opinion in Hematology·Jan J Schuringa, Edo Vellenga
Dec 17, 2009·Genome Research·Bas van SteenselTrey Ideker
Nov 24, 2011·Genome Research·Wenli ZhangJiming Jiang
Oct 14, 2006·Genome Research·Maartje J VogelBas van Steensel
Nov 9, 2007·Genome Research·Pär G EngströmBoris Lenhard
Sep 29, 2009·Plant Physiology·Sang Yeol KimZ Renee Sung
Dec 17, 2009·The Plant Cell·Jean-Benoit F CharronXing Wang Deng
Dec 24, 2010·Journal of Gastroenterology and Hepatology·Daisy P F Tsang, Alfred S L Cheng
Mar 10, 2010·Molecular and Cellular Biology·Hans-Martin HerzAndreas Bergmann
Oct 16, 2007·Doklady. Biochemistry and Biophysics·N A TchurikovE D Moiseeva
Feb 24, 2009·Doklady. Biochemistry and Biophysics·E D MoiseevaN A Tchurikov
Oct 5, 2010·Doklady. Biochemistry and Biophysics·D V SosinN A Tchurikov
Aug 15, 2008·Cancer Research·Dorothea DouglasElizabeth R Lawlor
Dec 14, 2011·Stroke; a Journal of Cerebral Circulation·Roger P SimonDavid Henshall

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.