Genome-wide real-time PCR for West Nile virus reduces the false-negative rate and facilitates new strain discovery.

Journal of Virological Methods
James F PapinDirk P Dittmer

Abstract

West Nile virus (WNV) causes significant morbidity and mortality worldwide. Transplant and transfusion recipients as well as the elderly are particularly at risk. WNV shows strain variation from season to season and from locale to locale. This poses a significant problem for diagnosis. Most assays use a single primer pair to detect WNV by QPCR, and can fail to detect novel stains. To overcome this limitation, a genome-wide, multiple primer-based real-time QPCR assay was developed for WNV. The same assay can be used for quantitation, viral variant discovery as well as for amplification of the entire viral genome using a single annealing temperature. It improves upon routine diagnosis as well as facilitates laboratory investigations of the pathology of WNV.

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Citations

Jul 28, 2012·Vector Borne and Zoonotic Diseases·Philip M ArmstrongTheodore G Andreadis
Mar 3, 2012·Veterinary Research·Marina De FiletteNiek N Sanders
May 22, 2016·Infectious Diseases·Shoba David, Asha Mary Abraham
Jul 26, 2019·Virus Evolution·Sean M BialosukniaAlexander T Ciota
Oct 10, 2020·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Meghan W StarolisEleanor A Powell

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