journal cover

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library

Nature Biotechnology

Feb 19, 2014

Hiroko Koike-YusaKosuke Yusa

Abstract

Identification of genes influencing a phenotype of interest is frequently achieved through genetic screening by RNA interference (RNAi) or knockouts. However, RNAi may only achieve partial depletion of gene activity, and knockout-based screens are difficult in diploid mammalian cells. H...read more

Mentioned in this Paper

Subfamily lentivirinae
Genome
CRISPR-Cas Systems
Cosuppression
Enhanced S-Cone Syndrome
Genetic Screening (Procedure)
Genomics
Thioguanine
Biotechnology
Mutant
29
4
1381
1
1
Paper Details
References
  • References47
  • Citations217
12345
  • References47
  • Citations217
12345...

Similar Papers Found In These Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

RNAi & Gene Silencing

RNA Interference (RNAi) pathway is involved in post-transcriptional gene silencing, transcriptional silencing and epigenetic silencing as well as its use as a tool for forward genetics and therapeutics. Discover the latest research here.

Embryonic Neural Stem Cells

Embryonic neural stem cells can be used to investigate the effect of different toxins and molecules on the proliferation and differentiation of these cells. Discover the latest research of embryonic neural stem cells here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

Related Papers

© 2020 Meta ULC. All rights reserved

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library

Nature Biotechnology

Feb 19, 2014

Hiroko Koike-YusaKosuke Yusa

PMID: 24535568

DOI: 10.1038/nbt.2800

Abstract

Identification of genes influencing a phenotype of interest is frequently achieved through genetic screening by RNA interference (RNAi) or knockouts. However, RNAi may only achieve partial depletion of gene activity, and knockout-based screens are difficult in diploid mammalian cells. H...read more

Mentioned in this Paper

Subfamily lentivirinae
Genome
CRISPR-Cas Systems
Cosuppression
Enhanced S-Cone Syndrome
Genetic Screening (Procedure)
Genomics
Thioguanine
Biotechnology
Mutant
29
4
1381
1
1

Similar Papers Found In These Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

RNAi & Gene Silencing

RNA Interference (RNAi) pathway is involved in post-transcriptional gene silencing, transcriptional silencing and epigenetic silencing as well as its use as a tool for forward genetics and therapeutics. Discover the latest research here.

Related Papers

Science

Genetic screens in human cells using the CRISPR-Cas9 system

ScienceDecember 18, 2013
Tim WangEric S Lander
Paper Details
References
  • References47
  • Citations217
12345
  • References47
  • Citations217
12345...
/papers/genome-wide-recessive-genetic-screening-in/24535568