Genome-wide screen for Salmonella genes required for long-term systemic infection of the mouse

PLoS Pathogens
Trevor D LawleyDenise M Monack

Abstract

A microarray-based negative selection screen was performed to identify Salmonella enterica serovar Typhimurium (serovar Typhimurium) genes that contribute to long-term systemic infection in 129X1/SvJ (Nramp1(r)) mice. A high-complexity transposon-mutagenized library was used to infect mice intraperitoneally, and the selective disappearance of mutants was monitored after 7, 14, 21, and 28 d postinfection. One hundred and eighteen genes were identified to contribute to serovar Typhimurium infection of the spleens of mice by 28 d postinfection. The negatively selected mutants represent many known aspects of Salmonella physiology and pathogenesis, although the majority of the identified genes are of putative or unknown function. Approximately 30% of the negatively selected genes correspond to horizontally acquired regions such as those within Salmonella pathogenicity islands (SPI 1-5), prophages (Gifsy-1 and -2 and remnant), and the pSLT virulence plasmid. In addition, mutations in genes responsible for outer membrane structure and remodeling, such as LPS- and PhoP-regulated and fimbrial genes, were also selected against. Competitive index experiments demonstrated that the secreted SPI2 effectors SseK2 and SseJ as well as the SPI4 ...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C A LeeS Falkow
May 1, 1974·The Journal of Experimental Medicine·P B Carter, F M Collins
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·A J BäumlerF Heffron
Jan 1, 1996·Annual Review of Immunology·B D Jones, S Falkow
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·D M MonackS Falkow
Sep 19, 1997·Trends in Microbiology·E A Groisman, H Ochman
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·D HershA Zychlinsky
May 5, 1999·Current Biology : CB·J H BrumellB B Finlay
Feb 19, 2000·Current Opinion in Microbiology·F G SauerS J Hultgren
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jun 9, 2000·Molecular Microbiology·R A Kingsley, A J Bäumler
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K E Unsworth, D W Holden
Jul 19, 2000·The Journal of Experimental Medicine·D M MonackS Falkow
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·C M SassettiE J Rubin
Nov 1, 2001·Annual Review of Cell and Developmental Biology·J E Galán
Nov 2, 2001·Nature Biotechnology·V BadarinarayanaG M Church
Jan 5, 2002·Microbes and Infection·R L SantosA J Bäumler
Nov 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michelle L ZaharikB Brett Finlay

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Citations

Mar 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·David S WeissDenise M Monack
Sep 11, 2014·Annual Review of Microbiology·Gordon Dougan, Stephen Baker
Dec 19, 2006·PLoS Genetics·Abram GabrielMaitreya J Dunham
Dec 26, 2006·Journal of Bacteriology·Erin C BoyleB Brett Finlay
Jan 31, 2007·Applied and Environmental Microbiology·Jetta J E BijlsmaOscar P Kuipers
Oct 9, 2007·Applied and Environmental Microbiology·Yanyan HuangCraig Altier
Oct 19, 2007·PLoS Genetics·Hany S GirgisSaeed Tavazoie
Nov 13, 2007·Journal of Bacteriology·Jeremy R Ellermeier, James M Slauch
Sep 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Víctor H BustamanteJosé L Puente
Feb 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Suzanne E OsborneBrian K Coombes
Aug 19, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mark P StevensDuncan J Maskell
Oct 3, 2009·Infection and Immunity·Veronica NovikJorge E Galán
Mar 2, 2010·Journal of Bacteriology·Supreet Saini, Christopher V Rao
Oct 19, 2010·Journal of Bacteriology·G Raja VenkateshKarin Schnetz
Feb 24, 2011·Infection and Immunity·Renée M TsolisAndreas J Bäumler
Mar 23, 2011·BMC Microbiology·Matthew R EvansHosni M Hassan
Aug 31, 2011·PLoS Pathogens·Maria FookesNicholas R Thomson

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Methods Mentioned

BETA
in vitro transcription
PCR

Software Mentioned

Excel
SMD
SAM ( Statistical Analysis of Microarrays
LACK
Access
Statistical Analysis of Microarrays ( SAM )
SAM
Treeview
Microarray Architect

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