Genome-wide transcriptional profiling of appressorium development by the rice blast fungus Magnaporthe oryzae.

PLoS Pathogens
Darren M SoanesN J Talbot

Abstract

The rice blast fungus Magnaporthe oryzae is one of the most significant pathogens affecting global food security. To cause rice blast disease the fungus elaborates a specialised infection structure called an appressorium. Here, we report genome wide transcriptional profile analysis of appressorium development using next generation sequencing (NGS). We performed both RNA-Seq and High-Throughput SuperSAGE analysis to compare the utility of these procedures for identifying differential gene expression in M. oryzae. We then analysed global patterns of gene expression during appressorium development. We show evidence for large-scale gene expression changes, highlighting the role of autophagy, lipid metabolism and melanin biosynthesis in appressorium differentiation. We reveal the role of the Pmk1 MAP kinase as a key global regulator of appressorium-associated gene expression. We also provide evidence for differential expression of transporter-encoding gene families and specific high level expression of genes involved in quinate uptake and utilization, consistent with pathogen-mediated perturbation of host metabolism during plant infection. When considered together, these data provide a comprehensive high-resolution analysis of gene ...Continue Reading

Associated Datasets

References

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Citations

Apr 16, 2014·Fungal Genetics and Biology : FG & B·Yue ChenZhengguang Zhang
Feb 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lauren S RyderNicholas J Talbot
Aug 2, 2013·Molecular Plant-microbe Interactions : MPMI·Aarón Rebollar, Belén López-García
Oct 1, 2013·Applied Microbiology and Biotechnology·Oliver Voigt, Stefanie Pöggeler
Jun 12, 2013·Annual Review of Phytopathology·Mihwa Yi, Barbara Valent
May 6, 2015·Molecular Genetics and Genomics : MGG·Dianguang XiongChengming Tian
Jan 15, 2015·Molecular Plant Pathology·Yue ChenZhengguang Zhang
Mar 12, 2015·BMC Genomics·Agustina LlanosJean-Luc Parrou
Mar 26, 2015·Plant Science : an International Journal of Experimental Plant Biology·Gea GuerrieroKhawar Sohail Siddiqui
May 14, 2016·Cellular and Molecular Life Sciences : CMLS·Van V Vu, Michael A Marletta
Jul 18, 2014·PLoS Pathogens·Daniel LanverRegine Kahmann
Feb 6, 2014·PLoS Pathogens·Daniel H ScharfAxel A Brakhage

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Datasets Mentioned

BETA
GSE30327

Methods Mentioned

BETA
RNA-Seq
four hybrid
scraping
PCR

Software Mentioned

Cufflinks
Tophat
FASTX Toolkit
MPSS
Bowtie
Blast2GO
FASTX
SuperSAGE
- Toolkit
FASTX Barcode Splitter

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