Genome-wide transcriptional response of an avian pathogenic Escherichia coli (APEC) pst mutant

BMC Genomics
Sébastien CrépinJosée Harel


Avian pathogenic E. coli (APEC) are associated with extraintestinal diseases in poultry. The pstSCAB-phoU operon belongs to the Pho regulon and encodes the phosphate specific transport (Pst) system. A functional Pst system is required for full virulence in APEC and other bacteria and contributes to resistance of APEC to serum, to cationic antimicrobial peptides and acid shock. The global mechanisms contributing to the attenuation and decreased resistance of the APEC pst mutant to environmental stresses have not been investigated at the transcriptional level. To determine the global effect of a pst mutation on gene expression, we compared the transcriptomes of APEC strain chi7122 and its isogenic pst mutant (K3) grown in phosphate-rich medium. Overall, 470 genes were differentially expressed by at least 1.5-fold. Interestingly, the pst mutant not only induced systems involved in phosphate acquisition and metabolism, despite phosphate availability, but also modulated stress response mechanisms. Indeed, transcriptional changes in genes associated with the general stress responses, including the oxidative stress response were among the major differences observed. Accordingly, the K3 strain was less resistant to reactive oxygen spec...Continue Reading


Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R A VanBogelen, F C Neidhardt
Aug 1, 1995·Veterinary Microbiology·C M DozoisJ M Fairbrother
Aug 1, 1996·Journal of Bacteriology·R A VanBogelenF C Neidhardt
Feb 26, 1998·Cell·B Bukau, A L Horwich
Nov 27, 1998·Canadian Journal of Microbiology·P C LoewenR Sparling
Mar 27, 1999·Journal of Bacteriology·E SuziedelienéS Normark
May 14, 1999·Current Opinion in Microbiology·G Storz, J A Imlay
Jan 11, 2000·Nucleic Acids Research·J D PetersonO White
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 5, 2002·Journal of Veterinary Pharmacology and Therapeutics·F BozićI Valpotić
May 25, 2002·Cellular Microbiology·Matthew A Mulvey
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Regine Hengge-Aronis
Dec 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Charles M DozoisRoy Curtiss
Feb 13, 2003·Nucleic Acids Research·Rafael A IrizarryTerence P Speed
Mar 26, 2004·Nature Reviews. Microbiology·James B KaperHarry L Mobley
Oct 21, 2004·Nature Reviews. Microbiology·John W Foster
Jun 15, 2005·Annual Review of Biochemistry·Deborah C JohnsonMichael K Johnson

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environmental stresses
electron microscopy
environmental stress

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