Genome-wide transcriptional response of an avian pathogenic Escherichia coli (APEC) pst mutant

BMC Genomics
Sébastien CrépinJosée Harel

Abstract

Avian pathogenic E. coli (APEC) are associated with extraintestinal diseases in poultry. The pstSCAB-phoU operon belongs to the Pho regulon and encodes the phosphate specific transport (Pst) system. A functional Pst system is required for full virulence in APEC and other bacteria and contributes to resistance of APEC to serum, to cationic antimicrobial peptides and acid shock. The global mechanisms contributing to the attenuation and decreased resistance of the APEC pst mutant to environmental stresses have not been investigated at the transcriptional level. To determine the global effect of a pst mutation on gene expression, we compared the transcriptomes of APEC strain chi7122 and its isogenic pst mutant (K3) grown in phosphate-rich medium. Overall, 470 genes were differentially expressed by at least 1.5-fold. Interestingly, the pst mutant not only induced systems involved in phosphate acquisition and metabolism, despite phosphate availability, but also modulated stress response mechanisms. Indeed, transcriptional changes in genes associated with the general stress responses, including the oxidative stress response were among the major differences observed. Accordingly, the K3 strain was less resistant to reactive oxygen spec...Continue Reading

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Citations

Nov 26, 2009·Current Microbiology·Martin G Lamarche, Josée Harel
Jun 19, 2012·Applied and Environmental Microbiology·Sébastien CrépinCharles M Dozois
May 23, 2014·Journal of Proteomics·Vanessa Rodrigues PegosAndrea Balan
Jun 28, 2011·Veterinary Microbiology·Sébastien CrépinJosée Harel
Mar 24, 2016·Molecular Plant-microbe Interactions : MPMI·Tiyakhon ChatnaparatSteven E Lindow
Aug 13, 2017·Applied and Environmental Microbiology·Tom J OverbeckJeff R Broadbent
Dec 9, 2014·Virulence·Samuel Mohammed ChekababCharles M Dozois
Sep 4, 2020·The EMBO Journal·Changhan LeeJames Ca Bardwell
Dec 7, 2018·Journal of Comparative Pathology·F F ArgentaS P Pavarini
Sep 15, 2019·EcoSal Plus·Stewart G Gardner, William R McCleary

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Datasets Mentioned

BETA
GSE9178

Methods Mentioned

BETA
environmental stresses
PCR
electrophoresis
electron microscopy
phosphotransferase
environmental stress

Software Mentioned

PrimerQuest
DNA Pattern
FlexArray
Colibri

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