Genome wide transcriptome analysis provides bases on colonic mucosal immune system development affected by colostrum feeding strategies in neonatal calves

BMC Genomics
Zhixiong HeLeluo Guan

Abstract

Delivery of colostrum within the first several hours after birth is vital for establishing successful passive immunity in neonatal dairy calves. However, it is unclear whether a difference in colostrum feeding strategy can affect the development of the calf gastrointestinal tract. The aim of this study was to evaluate the effect of colostrum feeding time within the first 12 h after birth on the colonic mucosal immune system in neonatal calves using a genome wide transcriptome analysis. RNA sequencing-based transcriptome analysis of colon tissues collected from 27 male Holstein calves which were randomly assigned to one of three colostrum feeding strategies - (immediately after birth (TRT0); 6 h after birth (TRT6); 12 h after birth (TRT12)) - and euthanized at 51 h of age detected 15,935 ± 210, 15,332 ± 415, and 15,539 ± 440 expressed genes in the colon under three treatments, respectively. The core transcriptome of the colon included 12,678 genes, with enriched "cellular process" and "metabolic process" as the top two biological functions with 802 of them being immune function related genes. Principal component analysis of the colon transcriptomes did not display a clear separation by colostrum feeding strategy and differential...Continue Reading

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Methods Mentioned

BETA
dissection
PCR
Assay
PCA
RNA-seq

Software Mentioned

PANTHER
Samtools
ImmPort
Weighted Gene Co - expression Network Analysis ( WGCNA
WGCNA
R
HTSeq
Tophat
count
ENSEMBL

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