Genomewide analysis of the lateral organ boundaries domain gene family in Eucalyptus grandis reveals members that differentially impact secondary growth

Plant Biotechnology Journal
Qiang LuDeyou Qiu

Abstract

Lateral Organ Boundaries Domain (LBD) proteins are plant-specific transcription factors playing crucial roles in growth and development. However, the function of LBD proteins in Eucalyptus grandis remains largely unexplored. In this study, LBD genes in E. grandis were identified and characterized using bioinformatics approaches. Gene expression patterns in various tissues and the transcriptional responses of EgLBDs to exogenous hormones were determined by qRT-PCR. Functions of the selected EgLBDs were studied by ectopically overexpressing in a hybrid poplar (Populus alba × Populus glandulosa). Expression levels of genes in the transgenic plants were investigated by RNA-seq. Our results showed that there were forty-six EgLBD members in the E. grandis genome and three EgLBDs displayed xylem- (EgLBD29) or phloem-preferential expression (EgLBD22 and EgLBD37). Confocal microscopy indicated that EgLBD22, EgLBD29 and EgLBD37 were localized to the nucleus. Furthermore, we found that EgLBD22, EgLBD29 and EgLBD37 were responsive to the treatments of indol-3-acetic acid and gibberellic acid. More importantly, we demonstrated EgLBDs exerted different influences on secondary growth. Namely, 35S::EgLBD37 led to significantly increased second...Continue Reading

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Citations

Apr 1, 2020·International Journal of Molecular Sciences·Yuwen ZhangXiangyuan Wan
Nov 6, 2018·International Journal of Molecular Sciences·Fenjuan ShaoDeyou Qiu
Oct 31, 2019·International Journal of Molecular Sciences·Hengzhi LiuQin Chen
Jun 16, 2021·Frontiers in Plant Science·Demetrio MarcianòSilvia Laura Toffolatti
Jul 28, 2021·Journal of Experimental Botany·Zhen HanLi-Jun Liu

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Methods Mentioned

BETA
interaction prediction
transgenic
transgenics
light
scraping
electrophoresis
PCR

Software Mentioned

Gene Structure Display Server
GOseq R package
Conserved Domain Architecture Retrieval Tool )
MEGA5
MEME
RSEM
STRING
SMART
ExPASy
cBot Cluster Generation System

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