Genomewide expression analysis in zebrafish mind bomb alleles with pancreas defects of different severity identifies putative Notch responsive genes.

PloS One
Ashok HegdeYun-Jin Jiang

Abstract

Notch signaling is an evolutionarily conserved developmental pathway. Zebrafish mind bomb (mib) mutants carry mutations on mib gene, which encodes a RING E3 ligase required for Notch activation via Delta/Jagged ubiquitylation and internalization. We examined the mib mutants for defects in pancreas development using in situ hybridization and GFP expression analysis of pancreas-specific GFP lines, carried out the global gene expression profile analysis of three different mib mutant alleles and validated the microarray data using real-time PCR and fluorescent double in situ hybridization. Our study showed that the mib mutants have diminished exocrine pancreas and this defect was most severe in mib(ta52b) followed by mib(m132) and then mib(tfi91), which is consistent with the compromised Notch activity found in corresponding mib mutant alleles. Global expression profile analysis of mib mutants showed that there is a significant difference in gene expression profile of wt and three mib mutant alleles. There are 91 differentially expressed genes that are common to all three mib alleles. Through detailed analysis of microarray data, we have identified several previously characterized genes and some putative Notch-responsive genes invo...Continue Reading

References

Sep 28, 1995·Nature·S JarriaultA Israel
Jan 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A InoueH Okamoto
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·F M Lu, S E Lux
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·R KopanJ S Nye
Dec 1, 1996·Development·M Furutani-SeikiC Nüsslein-Volhard
Apr 7, 1997·Biochemical and Biophysical Research Communications·J M PleauM Dardenne
Jul 1, 1997·Nature Genetics·T OdaS C Chandrasekharappa
May 30, 1998·Cell·G Struhl, A Adachi
Jul 4, 1998·Current Biology : CB·M Lecourtois, F Schweisguth
Jul 24, 1998·Trends in Biotechnology·M SchenaR W Davis
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Mar 13, 1999·Current Biology : CB·B AppelB B Riley
Apr 16, 1999·The EMBO Journal·T OhtsukaR Kageyama
Apr 30, 1999·Science·S Artavanis-TsakonasR J Lake
Jun 1, 1999·Methods in Enzymology·M B Eisen, P O Brown
Sep 7, 1999·Nature·A ApelqvistH Edlund
Dec 30, 1999·Nature Genetics·J JensenO D Madsen
Feb 1, 2000·Differentiation; Research in Biological Diversity·D LeakeN Schechter
Feb 9, 2000·Development Genes and Evolution·I RuvinskyR K Ho
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·G GradwohlF Guillemot
Aug 15, 2000·The Journal of Biological Chemistry·L QiuY C Liu
Nov 23, 2000·Genes & Development·R Kopan, A Goate
Dec 9, 2000·Developmental Biology·J S Mumm, R Kopan
Mar 22, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Y BesshoR Kageyama
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Jul 31, 2001·Genesis : the Journal of Genetics and Development·H HuangS Lin
Sep 7, 2001·Current Opinion in Cell Biology·M E Fortini
Jan 31, 2002·Current Biology : CB·Eric C Lai
Feb 7, 2002·Journal of Dental Research·R Todd, D T W Wong
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Martin OlbrotArun Sharma
Jan 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·He YangE Terry Papoutsakis
Mar 15, 2003·Developmental Cell·Ming-Ko Chiang, Douglas A Melton

❮ Previous
Next ❯

Citations

Oct 15, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gabriela A HortopanScott C Baraban
Nov 14, 2008·Molecular Genetics and Genomics : MGG·Junmo HwangSanggyu Lee
Aug 8, 2012·Nature Reviews. Genetics·K G GuruharshaSpyros Artavanis-Tsakonas
Jun 29, 2010·Trends in Genetics : TIG·Edor KabashiPierre Drapeau
Feb 11, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Mary D Kinkel, Victoria E Prince
Sep 2, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Michael J F BarresiRolf O Karlstrom
Jun 21, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Gabriela A Hortopan, Scott C Baraban
Nov 9, 2018·Antioxidants·Christine RamponSophie Vriz
Oct 5, 2010·Biochimica Et Biophysica Acta·Edor KabashiPierre Drapeau

❮ Previous
Next ❯

Datasets Mentioned

BETA
BI839927
AB259590
AF180921
GSE8522

Methods Mentioned

BETA
PCR
Chip
PCRs
chips
two-hybrid
transgenic
electrophoresis

Software Mentioned

GenePix Pro
TreeView
Significance Analysis of Microarrays ( SAM )
SPS
PERL
IPA
HomoloGene
Ingenuity Pathways Analysis ( IPA )
PERL script
SAM

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.