Genome‑wide identification of long noncoding RNAs in CCl4‑induced liver fibrosis via RNA sequencing

Molecular Medicine Reports
Zhenghua GongGuoxin Hu

Abstract

Liver fibrosis occurs as a result of chronic liver lesions, which may subsequently develop into liver cirrhosis and hepatocellular carcinoma. The involvement of long noncoding RNAs (lncRNAs) in liver fibrosis is being increasingly recognized. However, the exact mechanisms and functions of the majority of lncRNAs are poorly characterized. In the present study, the hepatotoxic substance carbon tetrachloride (CCl4) was employed to induce liver fibrosis in an animal model and agenome‑wide identification of lncRNAs in fibrotic liver tissues compared with CCl4 untreated liver tissues was performed using RNA sequencing. Sprague‑Dawley rats were treated with CCl4 for 8 weeks. Histopathogical alterations were observed in liver tissues, and serum levels of alanine aminotransferase, aspartate aminotransferase, transforming growth factor‑β1 and tumor necrosis factor‑α were significantly higher, in the CCl4‑treated group compared with the CCl4 untreated group. RNA sequencing of liver tissues demonstrated that 231 lncRNAs and 1,036 mRNAs were differentially expressed between the two groups. Furthermore, bioinformatics analysis demonstrated that the differentially expressed mRNAs were predominantly enriched in 'ECM‑receptor interaction', 'PI3...Continue Reading

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Citations

Mar 29, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Wei ChenHong You
Feb 27, 2020·International Journal of Molecular Sciences·Zhi HeMingyao Yang
Apr 4, 2019·Frontiers in Physiology·Siti Aishah SulaimanRahman Jamal

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Methods Mentioned

BETA
RNA-Seq

Software Mentioned

Cufflinks
SPSS
TopHat
gfold

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