Genomewide Transcriptional Responses of Iron-Starved Chlamydia trachomatis Reveal Prioritization of Metabolic Precursor Synthesis over Protein Translation

MSystems
Amanda J BrinkworthRey A Carabeo

Abstract

Iron is essential for growth and development of Chlamydia. Its long-term starvation in cultured mammalian cells leads to production of aberrant noninfectious chlamydial forms, also known as persistence. Immediate transcriptional responses to iron limitation have not been characterized, leaving a knowledge gap of how Chlamydia regulates its response to changes in iron availability. We used the fast-chelating agent 2,2'-bipyridyl (BPDL) to homogeneously starve Chlamydia trachomatis serovar L2 of iron, starting at 6 or 12 h postinfection. Immediate transcriptional responses were monitored after only 3 or 6 h of BPDL treatment, well before formation of aberrant Chlamydia. The first genomewide transcriptional response of C. trachomatis to iron starvation was subsequently determined utilizing RNA sequencing. Only 7% and 8% of the genome were differentially expressed in response to iron starvation at the early and middle stages of development, respectively. Biological pathway analysis revealed an overarching theme. Synthesis of macromolecular precursors (deoxynucleotides, amino acids, charged tRNAs, and acetyl coenzyme A [acetyl-CoA]) was upregulated, while energy-expensive processes (ABC transport and translation) were downregulated....Continue Reading

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Citations

Jan 9, 2019·Frontiers in Microbiology·Maria Emilia PanzettaHector Alex Saka
May 19, 2021·Infection and Immunity·Mary R Brockett, George W Liechti

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Datasets Mentioned

BETA
GSE106763

Methods Mentioned

BETA
confocal microscopy
light microscopy
PCR
chip
RNA-seq
chips
lipoylation
immunoprecipitation
proteomic profiling

Software Mentioned

CLC Genomics experiment
Rstudio
PANTHER
Affinity Designer
STRING
Torrent Suite
db
CLC Genomics
StringDB
CLC Genomics Workbench

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