Genomic analyses of a novel bacteriophage (VB_PmiS-Isfahan) within Siphoviridae family infecting Proteus mirabilis

Genomics
Mahsa YazdiEzzat Allah Ghaemi

Abstract

Proteus mirabilis is one of the most common causes of complicated urinary tract infections (UTI), especially in catheter-associated UTIs. The increased resistance to antibiotics, among P. mirabilis isolates has led us to search for alternative antibacterial agents. In this study, genome of a lytic Proteus phage VB_PmiS-Isfahan, isolated from wastewater, and active against planktonic and biofilms of P. mirabilis, isolated from UTI, was analyzed. Accordingly, the genome was sequenced and its similarity to other phages was assessed by the Mauve and EasyFig softwares. "One Click" was used for phylogenetic tree construction. The complete genome of VB_PmiS-Isfahan was 54,836 bp, dsDNA with a G+C content of 36.09%. Nighty-one open reading frames (ORFs) was deduced, among them, 23 were considered as functional genes, based on the homology to the previously characterized proteins. The BLASTn of VB_PmiS-Isfahan showed low similarity to complete genome of Salmonella phages VB_SenS_Sasha, 9NA, and VB_SenS-Sergei. A comparison of Nucleic acid and amino acid sequence, and phylogenetic analyses indicated that the phage is novel, significantly differs, and is distant from other genera, within Siphoviridae family. No virulence-associated and an...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 10, 2003·Journal of Clinical Microbiology·Noriyuki NaganoYoshichika Arakawa
Nov 24, 2006·Journal of Bacteriology·Tetsuyoshi InoueKazuhiro Fukui
Nov 6, 2008·Nucleic Acids Research·Patricia P Chan, Todd M Lowe
Nov 8, 2008·Bioinformatics·Tim CarverJulian Parkhill
Mar 3, 2009·Genome Research·Jared T SimpsonInanç Birol
Feb 1, 2011·Bioinformatics·Mitchell J SullivanScott A Beatson
May 11, 2012·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Chi-Yu ChenChun-Yu Lin
Jun 15, 2014·Archives of Virology·Seyed Mahdi GhasemiHyo-Ihl Chang
Nov 7, 2015·Microbiology Spectrum·Jessica N Schaffer, Melanie M Pearson
Dec 30, 2015·Antimicrobial Agents and Chemotherapy·Jonathan NzakizwanayoBrian V Jones
May 6, 2016·Frontiers in Microbiology·Carlota BardinaMontserrat Llagostera
Apr 7, 2017·Intervirology·Ira Aryani WirjonYahya Mat Arip

❮ Previous
Next ❯

Citations

Aug 30, 2019·Journal of Virology·Chi ZengSherwood R Casjens
Apr 28, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mahnaz Shafaei FallahEzzat Allah Ghaemi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Medycyna doświadczalna i mikrobiologia
K Izdebska-Szymona, E Zelazowska
Zeitschrift für allgemeine Mikrobiologie
R Geuther
Microbiology Resource Announcements
Taylor A BourgeoisMei Liu
Cahiers R. M. F. : bulletin médical-guide du praticien-revue médicale française
RENE-MARTIN de PRAINGY
© 2022 Meta ULC. All rights reserved