Genomic analysis and gene structure of the plant carotenoid dioxygenase 4 family: a deeper study in Crocus sativus and its allies

Genomics
Oussama AhrazemLourdes Gómez-Gómez

Abstract

The plastoglobule-targeted enzyme carotenoid cleavage dioxygenase (CCD4) mediates the formation of volatile C13 ketones, such as β-ionone, by cleaving the C9-C10 and C9'-C10' double bonds of cyclic carotenoids. Here, we report the isolation and analysis of CCD4 genomic DNA regions in Crocus sativus. Different CCD4 alleles have been identified: CsCCD4a which is found with and without an intron and CsCCD4b that showed the presence of a unique intron. The presence of different CCD4 alleles was also observed in other Crocus species. Furthermore, comparison of the locations of CCD4 introns within the coding region with CCD4 genes from other plant species suggests that independent gain/losses have occurred. The comparison of the promoter region of CsCCD4a and CsCCD4b with available CCD4 gene promoters from other plant species highlighted the conservation of cis-elements involved in light response, heat stress, as well as the absence and unique presence of cis-elements involved in circadian regulation and low temperature responses, respectively. Functional characterization of the Crocus sativus CCD4a promoter using Arabidopsis plants stably transformed with a DNA fragment of 1400 base pairs (P-CsCCD4a) fused to the β-glucuronidase (GU...Continue Reading

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Citations

Sep 6, 2013·Journal of Experimental Botany·María J RodrigoLorenzo Zacarías
Aug 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sarah FruscianteGiovanni Giuliano
Jun 19, 2012·BMC Genomics·Philip R YoungMelané A Vivier
Nov 22, 2011·BMC Plant Biology·Sabine GuillaumieSerge Delrot
Apr 5, 2012·Comparative and Functional Genomics·Hongli CuiSong Qin
Sep 29, 2015·Journal of Agricultural and Food Chemistry·Oussama AhrazemLourdes Gómez-Gómez
May 9, 2015·The New Phytologist·Carolyn A Wessinger
Mar 10, 2015·Molecular Genetics and Genomics : MGG·Xiongjie ZhengZhiyong Pan
Jun 28, 2016·Trends in Plant Science·Xin HouBarry J Pogson
Oct 30, 2016·International Journal of Molecular Sciences·Oussama AhrazemMaría Carmen Limón
Oct 6, 2017·Journal of Experimental Botany·Oussama AhrazemLourdes Gómez-Gómez
Dec 27, 2019·Briefings in Functional Genomics·Manoj Kumar DharSanjana Kaul
Oct 28, 2019·Horticulture Research·Jerry LinDario Cantu
May 12, 2018·International Journal of Molecular Sciences·Lourdes Gómez-GómezOussama Ahrazem
Jun 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·John J B TimminsIsak S Pretorius
Dec 27, 2016·Frontiers in Plant Science·Sarah RottetFelix Kessler
Apr 9, 2021·Frontiers in Plant Science·Markus RienthSimone Diego Castellarin
Jul 29, 2021·Plant Science : an International Journal of Experimental Plant Biology·Ressin VargheseSiva Ramamoorthy

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