Genomic analysis of Blastocystis hominis isolates in patients with HIV-positive using locus SSU-rDNA

Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology
Anahita Razavi PiranshahiShahram Khademvatan

Abstract

Blastocystis hominis (B. hominis) is a protozoan zoonosis which clinical signs of infection with this parasite has been reported to be more severe in patients with weakened immune systems than healthy controls. So, the aim of the study was to evaluate genomic analysis of B. hominis isolates obtained from patients with HIV-positive using locus SSU-rDNA. At first, 268 stool samples were randomly collected from patients with HIV-positive referred to health centers of Khuzestan province, southwest of Iran. Formol-ether and direct smear techniques were used for the detection of parasitic agents. After extracting DNA, the samples were analyzed by the PCR method. Finally, the subtypes were determined by the sequencing and PCR methods. New samples were used for the preparation of positive control sample; they were cultured in coagulant-serum biphasic cultivation media. Of 268 stool samples, 33 (12.3%) cases were detected positive for B. hominis using Formol-Ether technique but 51 (19%) cases were positive using molecular method. The most common isolates were related to the subtype III with 29 positive cases (56.8%), then, genotype I with 11 (21.6%) cases, 6 cases (11.8%) with genotype II, 3 (5.9%) combined cases with genotypes I and II...Continue Reading

References

Jul 22, 1999·European Journal of Epidemiology·O CirioniG Scalise
Apr 4, 2000·Journal of Clinical Microbiology·H YoshikawaY Takahashi
Apr 14, 2000·Journal of Gastroenterology and Hepatology·M E HellardC K Fairley
Jun 28, 2000·European Journal of Epidemiology·S GuignardH Rubinstein
Jan 24, 2007·Trends in Parasitology·C Rune StensvoldC Graham Clark
Jan 12, 2008·Annals of Tropical Medicine and Parasitology·H MotazedianS M Sadjjadi
Aug 30, 2008·International Journal for Parasitology·C Rune StensvoldC Graham Clark
Aug 18, 2009·Parasitology Research·Fadime ErogluIsmail Soner Koltas
Jan 22, 2010·Veterinary Parasitology·Unaiza ParkarR C Andrew Thompson
Nov 4, 2011·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Xu ZhangZhichao Wei
May 13, 2015·Jundishapur Journal of Microbiology·Ebrahim BadparvaFarnaz Kheirandish

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