Genomic analysis of Klebsiella pneumoniae isolates from Malawi reveals acquisition of multiple ESBL determinants across diverse lineages.

The Journal of Antimicrobial Chemotherapy
Patrick MusichaNicholas Feasey

Abstract

ESBL-producing Klebsiella pneumoniae (KPN) pose a major threat to human health globally. We carried out a WGS study to understand the genetic background of ESBL-producing KPN in Malawi and place them in the context of other global isolates. We sequenced genomes of 72 invasive and carriage KPN isolates collected from patients admitted to Queen Elizabeth Central Hospital, Blantyre, Malawi. We performed phylogenetic and population structure analyses on these and previously published genomes from Kenya (n = 66) and from outside sub-Saharan Africa (n = 67). We screened for presence of antimicrobial resistance (AMR) genetic determinants and carried out association analyses by genomic sequence cluster, AMR phenotype and time. Malawian isolates fit within the global population structure of KPN, clustering into the major lineages of KpI, KpII and KpIII. KpI isolates from Malawi were more related to those from Kenya, with both collections exhibiting more clonality than isolates from the rest of the world. We identified multiple ESBL genes, including blaCTX-M-15, several blaSHV, blaTEM-63 and blaOXA-10, and other AMR genes, across diverse lineages of the KPN isolates from Malawi. No carbapenem resistance genes were detected; however, we d...Continue Reading

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Jan 29, 2019·The Journal of Antimicrobial Chemotherapy·Christopher T WilliamsThomas Edwards
Sep 16, 2020·The Journal of Antimicrobial Chemotherapy·Geoffrey Peterkins KumwendaShigeyuki Hamada
Feb 15, 2020·Nature Reviews. Microbiology·Kelly L WyresKathryn E Holt

Datasets Mentioned

BETA
CP022127
AP006725
JN420336.1

Related Concepts

Bacteriocidal Agents
Beta-Lactamase
Homo sapiens
Klebsiella Infections
Klebsiella rhinoscleromatis
Malawi
Breakpoint Determination, Antibacterial Susceptibility
Phylogeny
Variation (Genetics)
Genome, Bacterial

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