Genomic Analysis of Multiresistant Staphylococcus capitis Associated with Neonatal Sepsis

Antimicrobial Agents and Chemotherapy
Glen P CarterDeborah A Williamson

Abstract

Coagulase-negative staphylococci (CoNS), such as Staphylococcus capitis, are major causes of bloodstream infections in neonatal intensive care units (NICUs). Recently, a distinct clone of S. capitis (designated S. capitis NRCS-A) has emerged as an important pathogen in NICUs internationally. Here, 122 S. capitis isolates from New Zealand (NZ) underwent whole-genome sequencing (WGS), and these data were supplemented with publicly available S. capitis sequence reads. Phylogenetic and comparative genomic analyses were performed, as were phenotypic assessments of antimicrobial resistance, biofilm formation, and plasmid segregational stability on representative isolates. A distinct lineage of S. capitis was identified in NZ associated with neonates and the NICU environment. Isolates from this lineage produced increased levels of biofilm, displayed higher levels of tolerance to chlorhexidine, and were multidrug resistant. Although similar to globally circulating NICU-associated S. capitis strains at a core-genome level, NZ NICU S. capitis isolates carried a novel stably maintained multidrug-resistant plasmid that was not present in non-NICU isolates. Neonatal blood culture isolates were indistinguishable from environmental S. capitis...Continue Reading

References

Sep 30, 2010·Archives of Disease in Childhood. Fetal and Neonatal Edition·Stefania VergnanoPaul T Heath
Jul 27, 2012·The Journal of Antimicrobial Chemotherapy·Carolyne HornerMark Wilcox
Oct 13, 2012·Frontiers in Cellular and Infection Microbiology·David CueChia Y Lee
Feb 15, 2013·Molecular Biology and Evolution·Lu ChengJukka Corander
Dec 24, 2013·Journal of Bacteriology·Alice G ChengOlaf Schneewind
Mar 5, 2014·Antimicrobial Resistance and Infection Control·Julie HartJ Owen Robinson
Aug 21, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Deborah A WilliamsonJohn D Fraser
Nov 6, 2014·Molecular Biology and Evolution·Lam-Tung NguyenBui Quang Minh
Jul 24, 2015·The Journal of Antimicrobial Chemotherapy·M ButinJ P Rasigade
Sep 26, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M ButinF Laurent
May 5, 2016·Nucleic Acids Research·David ArndtDavid S Wishart
Jul 19, 2016·The Journal of Hospital Infection·M Ben SaidJ-C Picaud
Feb 9, 2017·The New England Journal of Medicine·Deborah WilliamsonTimothy Stinear
Feb 22, 2017·Emerging Infectious Diseases·Marine ButinFrédéric Laurent
Feb 2, 2018·Nature Ecology & Evolution·Fernando Domingues Kümmel TriaTal Dagan

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Citations

Sep 29, 2019·Applied and Environmental Microbiology·Bárbara DuarteCarla Novais
Nov 2, 2018·Journal of Clinical Microbiology·Shannon KatiyoGauri Godbole
Jun 11, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Marie DecalonneNathalie Van Der Mee-Marquet
Aug 10, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Bianca StenmarkBo Söderquist
Apr 29, 2020·Nature Microbiology·Thierry WirthUNKNOWN ESGS Study Group of ESCMID
Oct 23, 2019·Antimicrobial Resistance and Infection Control·Marine ButinFrédéric Laurent
Feb 26, 2021·Pediatric Research·Marie ChavignonMarine Butin
Jun 1, 2021·Frontiers in Immunology·Cristina KalbermatterStephanie C Ganal-Vonarburg
Apr 22, 2020·American Journal of Infection Control·Yoonkyo OhGina Lim

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

hierBAPS
Gubbins
BLAST
Nullarbor
Etest

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