PMID: 2122000Nov 5, 1990Paper

Genomic analysis of sequence variation in tandemly repeated DNA. Evidence for localized homogeneous sequence domains within arrays of alpha-satellite DNA

Journal of Molecular Biology
P E Warburton, H F Willard

Abstract

As a model to examine the local distribution of sequence variation within large arrays of tandemly repeated DNA in complex genomes, the long-range organization of alpha-satellite DNA from human chromosome 17 was investigated. Three individual chromosomes, representing different alpha-satellite haplotypes, were segregated into mouse and human somatic cell hybrids and their arrays sized by pulse-field gel electrophoresis. An inventory of the higher-order repeat units found in multiple separate regions of these megabase arrays was obtained using cosmid mapping and two-dimensional gel electrophoresis, a technique that combines the large-scale resolution of pulsed-field gel electrophoresis with the small-scale resolution of conventional gel electrophoresis. These analyses show that alpha-satellite arrays are characterized by the presence of localized homogeneous domains containing only one distinct type of repeat unit. These domains, which consist of sequence variants and/or higher-order repeat length variants, can be up to at least several hundred thousands of bases in length. Both abundant and rare variant repeat units can be localized in these distinct domains, which may correspond to transition states in the evolution of tandem ...Continue Reading

References

Dec 5, 1979·Journal of Molecular Biology·M Carlson, D Brutlag
Nov 1, 1979·Cell·M PechH G Zachau
Jul 15, 1976·Nature·H Cooke
May 5, 1975·Journal of Molecular Biology·E M Southern
Jun 5, 1987·Journal of Molecular Biology·C Tyler-Smith, W R Brown
Nov 5, 1985·Journal of Molecular Biology·R A Buckland, J K Elder
Feb 25, 1987·Nucleic Acids Research·H VissingR Williamson
Mar 20, 1987·Journal of Molecular Biology·A R Lohe, D L Brutlag
Jan 1, 1988·Chromosoma·I A AlexandrovY B Yurov
May 10, 1980·Nucleic Acids Research·N L BrownP R Whitehead
Sep 25, 1980·Journal of Molecular Biology·J C Wu, L Manuelidis
Jan 1, 1980·Annual Review of Genetics·D L Brutlag
Oct 25, 1982·Journal of Molecular Biology·T N Lee, M F Singer
Jan 25, 1984·Nucleic Acids Research·D A EpsteinA V Furano
Jul 1, 1984·Cell·R T Schimke
Oct 1, 1984·Genetics·T Ohta, G A Dover

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Citations

Nov 1, 1993·Journal of Molecular Evolution·G M GreigH F Willard
Oct 1, 1995·Chromosoma·S NarayanswamiB A Hamkalo
May 1, 1993·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·T HaafH F Willard
Jan 1, 1994·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·J L FernándezJ Gosálvez
Aug 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·A Losada, A Villasante
Jun 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·R MüllenbachN Blin
Aug 1, 1993·Journal of Molecular Evolution·B MarçaisG Roizès
May 11, 2005·Journal of Molecular Evolution·Brankica MravinacMiroslav Plohl
Oct 1, 1992·Trends in Genetics : TIG·S M Williams, L G Robbins
May 21, 2013·Trends in Genetics : TIG·Catarina D Campbell, Evan E Eichler
Dec 1, 1991·Current Opinion in Genetics & Development·H F Willard
Aug 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kristin A MaloneyBeth A Sullivan
Aug 11, 2011·Genome Biology and Evolution·Hye-Ran LeeHuntington F Willard
May 12, 2009·Molecular Biology and Evolution·A CellamareM Ventura
Dec 14, 2005·Genome Research·M Katharine RuddHuntington F Willard
Mar 5, 2010·Molekuliarnaia biologiia·E V EvtushenkoA V Vershinin
Jul 24, 2012·BMC Genomics·Karen E Hayden, Huntington F Willard
Nov 11, 2008·BMC Genomics·Peter E WarburtonGyorgy Abrusan
Sep 14, 2015·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Karen H Miga
Apr 14, 2017·Nucleus·Lori L SullivanBeth A Sullivan
Jan 1, 1996·Environmental and Molecular Mutagenesis·B A SullivanH F Willard
Sep 21, 2017·Genes·Manuel A Garrido-Ramos
Jun 1, 1994·Bio/technology·C HuxleyT Haaf
Apr 17, 2004·The Journal of Biological Chemistry·Allen ChiuRolf Jessberger
Jun 24, 2005·Gene Therapy·A LanerC Grillot-Courvalin

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