Genomic Analysis of Shewanella sp. O23S-The Natural Host of the pSheB Plasmid Carrying Genes for Arsenic Resistance and Dissimilatory Reduction

International Journal of Molecular Sciences
Witold UhrynowskiLukasz Drewniak

Abstract

Shewanella sp. O23S is a dissimilatory arsenate reducing bacterial strain involved in arsenic transformations within the abandoned gold mine in Zloty Stok (SW Poland). Previous physiological studies revealed that O23S may not only release arsenic from minerals, but also facilitate its immobilization through co-precipitation with reduced sulfur species. Given these uncommon, complementary characteristics and the application potential of the strain in arsenic-removal technologies, its genome (~5.3 Mbp), consisting of a single chromosome, two large plasmids (pSheA and pSheB) and three small plasmid-like phages (pSheC-E) was sequenced and annotated. Genes encoding putative proteins involved in heavy metal transformations, antibiotic resistance and other phenotypic traits were identified. An in-depth comparative analysis of arsenic respiration (arr) and resistance (ars) genes and their genetic context was also performed, revealing that pSheB carries the only copy of the arr genes, and a complete ars operon. The plasmid pSheB is therefore a unique natural vector of these genes, providing the host cells arsenic respiration and resistance abilities. The functionality of the identified genes was determined based on the results of the pr...Continue Reading

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Citations

Nov 28, 2019·Environmental Microbiology Reports·Timothy R McDermottRonald S Oremland
Sep 17, 2020·International Journal of Molecular Sciences·Katarzyna BujakMonika Radlinska
Oct 6, 2021·Letters in Applied Microbiology·L C StaicuJ L R Gallego

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Methods Mentioned

BETA
PCR
genetic modification
electrophoresis
transmission electron microscopy

Software Mentioned

Biolog
REBASE
Psi
Biolog TM
MUSCLE
PHAST
VIRFAM
[UNK] EcoPlate
Newbler de novo assembler
Artemis

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