PMID: 1347950Mar 15, 1992

Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts

Proceedings of the National Academy of Sciences of the United States of America
J M RossiS M Tilghman

Abstract

A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.

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Related Concepts

Genome
Restriction Fragment Length Polymorphism
Spleen
Polymerase Chain Reaction Analysis
Genetic Markers
Genome Mapping
Genomics
Genomic DNA
Chromosomes, Human, Pair 17
cDNA Library

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