Abstract
The hyperthermophilic archaeum, Pyrobaculum aerophilum, grows optimally at 100 degrees C with a doubling time of 180 min. It is a member of the phylogenetically ancient Thermoproteales order, but differs significantly from all other members by its facultatively aerobic metabolism. Due to its simple cultivation requirements and its nearly 100% plating efficiency, it was chosen as a model organism for studying the genome organization of hyperthermophilic ancient archaea. By a G+C content of the DNA of 52 mol%, sequence analysis was easily possible. At least some of the mRNA of P. aerophilum carried poly-A tails facilitating the construction of a cDNA library. 245 sequence tags of a poly-A primed cDNA library and 55 sequence tags from a 1-2 kb Sau3AI-fragment containing genomic library were analyzed and the corresponding amino acid sequences compared with protein sequences from databases. Fourteen percent of the cDNA and >9% of genomic DNA sequence tags revealed significant similarities to proteins in the databases. Matches were obtained to proteins from archaeal, bacterial and eukaryal sources. Some sequences showed greatest similarity to eukaryal rather than to bacterial versions of proteins, other matches were found to proteins...Continue Reading
References
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1992·Nature Genetics·C I Bargmann
May 1, 1992·Journal of Molecular Evolution·P CammaranoO Tiboni
Jun 21, 1991·Science·M D AdamsR F Moreno
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C R WoeseM L Wheelis
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1987·Analytical Biochemistry·P Chomczynski, N Sacchi
Jun 1, 1985·Journal of Bacteriology·J W Brown, J N Reeve
Jul 24, 1981·Nucleic Acids Research·Y GopalakrishnaN Sarkar
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·P J Keeling, W F Doolittle
Jun 20, 1993·Journal of Molecular Biology·C W KimJ H Miller
Sep 1, 1993·Applied and Environmental Microbiology·P VölklK O Stetter
Aug 1, 1994·Protein Science : a Publication of the Protein Society·P VölklJ H Miller
Apr 1, 1994·Molecular & General Genetics : MGG·C SchleperW Zillig
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S BurggrafK O Stetter
Aug 1, 1988·Applied and Environmental Microbiology·R J Sharp, R A Williams
Citations
Jun 26, 1999·Proteins·M PellegriniT O Yeates
Apr 30, 2003·Journal of Structural Biology·Celia W Goulding, L Jeanne Perry
Oct 3, 2002·RNA·Elisabetta BiniPaul Blum
Jan 13, 2000·European Journal of Biochemistry·V CuraD Kern
Dec 20, 2002·Nucleic Acids Research·Andreas JägerGabriele Klug
Jul 24, 2007·Journal of Bacteriology·Sonja HundtGabriele Klug
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Philip A. ReaEnrico Martinoia
May 24, 2001·Journal of Molecular Biology·M M SlupskaJ H Miller
May 17, 2017·Microorganisms·Annarita PoliBarbara Nicolaus
Jan 28, 1999·Journal of Bacteriology·S J SandlerA J Clark