PMID: 8948626Nov 15, 1996Paper

Genomic and cDNA sequence tags of the hyperthermophilic archaeon Pyrobaculum aerophilum

Nucleic Acids Research
P VölklJ H Miller

Abstract

The hyperthermophilic archaeum, Pyrobaculum aerophilum, grows optimally at 100 degrees C with a doubling time of 180 min. It is a member of the phylogenetically ancient Thermoproteales order, but differs significantly from all other members by its facultatively aerobic metabolism. Due to its simple cultivation requirements and its nearly 100% plating efficiency, it was chosen as a model organism for studying the genome organization of hyperthermophilic ancient archaea. By a G+C content of the DNA of 52 mol%, sequence analysis was easily possible. At least some of the mRNA of P. aerophilum carried poly-A tails facilitating the construction of a cDNA library. 245 sequence tags of a poly-A primed cDNA library and 55 sequence tags from a 1-2 kb Sau3AI-fragment containing genomic library were analyzed and the corresponding amino acid sequences compared with protein sequences from databases. Fourteen percent of the cDNA and >9% of genomic DNA sequence tags revealed significant similarities to proteins in the databases. Matches were obtained to proteins from archaeal, bacterial and eukaryal sources. Some sequences showed greatest similarity to eukaryal rather than to bacterial versions of proteins, other matches were found to proteins...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1992·Nature Genetics·C I Bargmann
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C R WoeseM L Wheelis
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jun 1, 1985·Journal of Bacteriology·J W Brown, J N Reeve
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·P J Keeling, W F Doolittle
Sep 1, 1993·Applied and Environmental Microbiology·P VölklK O Stetter
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S BurggrafK O Stetter

❮ Previous
Next ❯

Citations

Apr 30, 2003·Journal of Structural Biology·Celia W Goulding, L Jeanne Perry
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Philip A. ReaEnrico Martinoia
May 24, 2001·Journal of Molecular Biology·M M SlupskaJ H Miller
May 17, 2017·Microorganisms·Annarita PoliBarbara Nicolaus

❮ Previous
Next ❯

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.