Genomic and Clinical Characterization of IMP-1-Producing Multidrug-Resistant Acinetobacter bereziniae Isolates from Bloodstream Infections in a Brazilian Tertiary Hospital.

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Lais Calissi Brisolla TavaresCarlos Henrique Camargo

Abstract

Acinetobacter baumannii is the main species of the Acinetobacter genus; however, non-baumannii Acinetobacter (NBA) species causing infections have been described for the past years, as well as antimicrobial resistance. In this study, we describe the occurrence of two multidrug-resistant (MDR) IMP-1-producing Acinetobacter bereziniae isolates recovered from bloodstream infections in different patients but in the same intensive care unit among 134 carbapenem-resistant Acinetobacter screened. Antimicrobial susceptibility testing revealed resistance to carbapenems, extended spectrum, and antipseudomonad cephalosporins, amikacin, and trimethoprim-sulfamethoxazole. Both A. bereziniae isolates shared the same ApaI-pulsed-field gel electrophoresis (PFGE) pattern. Whole-genome sequencing of both isolates revealed that blaIMP-1 was embedded into an In86 Class I integron carrying also sul1, aac(6')-31, and aadA genes. A new sequence type (ST1309 Pasteur) was deposited. The virulence genes lpxC and ompA, seen in A. baumannii, were detected in the A. bereziniae strains. Recognition of A. bereziniae causing invasive MDR infection underscores the role of NBA species as human pathogens especially in at-risk patients.

References

Jan 1, 1991·Antimicrobial Agents and Chemotherapy·M WatanabeS Mitsuhashi
Mar 7, 2006·Journal of Clinical Microbiology·Bernard La ScolaDidier Raoult
Mar 28, 2006·International Journal of Antimicrobial Agents·Neil WoodfordDavid M Livermore
Jul 27, 2006·Internal Medicine Journal·A Y Peleg, D L Paterson
Aug 9, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Robert A Bonomo, Dora Szabo
Aug 18, 2006·BMC Infectious Diseases·Axel KramerGünter Kampf
Mar 25, 2008·International Journal of Antimicrobial Agents·Po-Liang LuL K Siu
May 1, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Lisa L Maragakis, Trish M Perl
Jul 16, 2008·Clinical Microbiology Reviews·Anton Y PelegDavid L Paterson
Jul 6, 2010·International Journal of Antimicrobial Agents·Kyungwon LeeYunsop Chong
Feb 1, 2011·Bioinformatics·Mitchell J SullivanScott A Beatson
Mar 15, 2011·Diagnostic Microbiology and Infectious Disease·Laurent PoirelPatrice Nordmann
Jul 29, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A-P MagiorakosD L Monnet
Sep 3, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R A BonninP Nordmann
Oct 15, 2011·International Journal of Antimicrobial Agents·Young Kyoung ParkKwan Soo Ko
Apr 18, 2012·Antimicrobial Agents and Chemotherapy·Rémy A BonninPatrice Nordmann
Nov 30, 2013·The Journal of Antimicrobial Chemotherapy·Witchuda KamolvitHarald Seifert
Dec 7, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R ZhangG-X Chen
Aug 8, 2015·Antimicrobial Agents and Chemotherapy·Marco BrovedanAdriana S Limansky
Oct 2, 2015·Current Drug Targets·Maria F MojicaWalter Fast
Oct 7, 2015·Future Microbiology·Filipa GrossoLuísa Peixe
Feb 13, 2016·Frontiers in Microbiology·Ahmad Al AtrouniMarie Kempf
Aug 16, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Mariana PaganoAfonso Luis Barth
Jun 1, 2015·Journal of Global Antimicrobial Resistance·Thiago Pavoni Gomes ChagasMarise Dutra Asensi
Feb 9, 2017·Antimicrobial Agents and Chemotherapy·Yasufumi MatsumuraJohann D D Pitout

❮ Previous
Next ❯

Citations

Dec 31, 2020·Antibiotics·Adam BarakaMaria Soledad Ramirez

❮ Previous
Next ❯

Datasets Mentioned

BETA
SIRG01000000

Methods Mentioned

BETA
PCR
electrophoresis
whole-genome shotgun sequencing
X-ray

Software Mentioned

BactDB
MLST
rMLST
Geneious
CSI Phylogeny
kmer
EasyFig
BioNumerics
ResFinder
Sensititre

Related Concepts

Related Feeds

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Acinetobacter Infections

Acinetobacter infections have become common in hospitalized patients, especially in the intensive care unit setting and are difficult to treat due to their propensity to develop antimicrobial drug resistance. Discover the latest research on Acinetobacter Infections here.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Related Papers

Journal of Global Antimicrobial Resistance
Thiago Pavoni Gomes ChagasMarise Dutra Asensi
Journal of Global Antimicrobial Resistance
Larissa Dos Santos FávaroFloristher Elaine Carrara-Marroni
© 2021 Meta ULC. All rights reserved