Genomic and functional characterisation of IncX3 plasmids encoding blaSHV-12 in Escherichia coli from human and animal origin

Scientific Reports
Apostolos LiakopoulosDik J Mevius

Abstract

The blaSHV-12 β-lactamase gene is one of the most prevalent genes conferring resistance to extended-spectrum β-lactams in Enterobacteriaceae disseminating within and between reservoirs, mostly via plasmid-mediated horizontal gene transfer. Yet, studies regarding the biology of plasmids encoding blaSHV-12 are very limited. In this study, we revealed the emergence of IncX3 plasmids alongside IncI1α/γ in blaSHV-12 in animal-related Escherichia coli isolates. Four representative blaSHV-12-encoding IncX3 plasmids were selected for genome sequencing and further genetic and functional characterization. We report here the first complete sequences of IncX3 plasmids of animal origin and show that IncX3 plasmids exhibit remarkable synteny in their backbone, while the major differences lie in their blaSHV-12-flanking region. Our findings indicate that plasmids of this subgroup are conjugative and highly stable, while they exert no fitness cost on their bacterial host. These favourable features might have contributed to the emergence of IncX3 amongst SHV-12-producing E. coli in the Netherlands, highlighting the epidemic potential of these plasmids.

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Citations

Feb 13, 2020·Open Forum Infectious Diseases·Tim FlerlageRandall Hayden
Mar 17, 2020·The Journal of Infectious Diseases·Baomo LiuChao Zhuo

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Datasets Mentioned

BETA
CP002910
CP008831
KX618696
KX618697
KX618698
KX618703

Methods Mentioned

BETA
PCR

Software Mentioned

SplitsTree4
BioNJ
RAST
R
BLAST
RStudio
MLST
BLASTP
Nucmer
ResFinder

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