Genomic and genetic analysis of Bordetella bacteriophages encoding reverse transcriptase-mediated tropism-switching cassettes

Journal of Bacteriology
Minghsun LiuJeff F Miller

Abstract

Liu et al. recently described a group of related temperate bacteriophages that infect Bordetella subspecies and undergo a unique template-dependent, reverse transcriptase-mediated tropism switching phenomenon (Liu et al., Science 295: 2091-2094, 2002). Tropism switching results from the introduction of single nucleotide substitutions at defined locations in the VR1 (variable region 1) segment of the mtd (major tropism determinant) gene, which determines specificity for receptors on host bacteria. In this report, we describe the complete nucleotide sequences of the 42.5- to 42.7-kb double-stranded DNA genomes of three related phage isolates and characterize two additional regions of variability. Forty-nine coding sequences were identified. Of these coding sequences, bbp36 contained VR2 (variable region 2), which is highly dynamic and consists of a variable number of identical 19-bp repeats separated by one of three 5-bp spacers, and bpm encodes a DNA adenine methylase with unusual site specificity and a homopolymer tract that functions as a hotspot for frameshift mutations. Morphological and sequence analysis suggests that these Bordetella phage are genetic hybrids of P22 and T7 family genomes, lending further support to the ide...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Mar 1, 1975·Virology·C RudolphS Stirm
Aug 1, 1990·Molecular & General Genetics : MGG·J L Zhao, C S Orser
Jun 1, 1988·Molecular & General Genetics : MGG·R Hausmann, M Messerschmid
Aug 1, 1982·Journal of Virology·B KwiatkowskiS Stirm
Jun 28, 1996·Science·M K Waldor, J J Mekalanos
Dec 1, 1996·Journal of Bacteriology·R LegouxM Salomé
Jul 1, 1997·Journal of Bacteriology·V MagriniP Youderian
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 4, 1999·Molecular Microbiology·I R HendersonJ P Nataro
Aug 5, 2000·Journal of Molecular Biology·M D ErmolaevaS L Salzberg
Aug 10, 2000·Molecular Microbiology·P S Rydman, D H Bamford
Oct 6, 2000·Annual Review of Microbiology·Peggy A Cotter, V J DiRita
Dec 20, 2000·Bioinformatics·K RutherfordBart Barrell
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·D NelsonV A Fischetti
Sep 7, 2001·Annual Review of Microbiology·W C Summers
Nov 2, 2001·Frontiers in Bioscience : a Journal and Virtual Library·S MattooJ F Miller
Jul 27, 2002·Journal of Bacteriology·Forest Rohwer, Rob Edwards
Apr 23, 2003·Cell·Marisa L PedullaGraham F Hatfull

Citations

Apr 10, 2013·Archives of Virology·Karin Moelling
Aug 20, 2008·Nature Biotechnology·Marina G KalyuzhnayaLudmila Chistoserdova
Sep 20, 2005·Nature Structural & Molecular Biology·Stephen A McMahonPartho Ghosh
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Wei DaiZ Hong Zhou
Apr 24, 2013·Molecular Biology and Evolution·Thomas W SchoenfeldBrian P Hedlund
Jan 31, 2012·Protein Engineering, Design & Selection : PEDS·Cathie M OverstreetGregory A Weiss
Jun 23, 2006·Proceedings. Biological Sciences·Jaewook JooEric Harvill
Dec 15, 2005·Journal of Bacteriology·Elizabeth J SummerRy Young
Nov 17, 2011·MBio·Philippe LehoursAnders F Andersson
Jul 20, 2012·Journal of Bioinformatics and Computational Biology·Irina AbnizovaTony Cox
Jun 23, 2006·PLoS Genetics·Graham F HatfullRoger W Hendrix
Dec 24, 2011·PLoS Genetics·Huatao GuoJeff F Miller
Apr 1, 2011·Cellular and Molecular Life Sciences : CMLS·Lenka Pilousova, Ivan Rychlik
Aug 21, 2007·Current Opinion in Microbiology·Bob Medhekar, Jeff F Miller
Sep 25, 2007·Research in Microbiology·Hans-W Ackermann, Andrew M Kropinski
Oct 23, 2009·Annals of the New York Academy of Sciences·Luis P Villarreal
Aug 15, 2009·Environmental Microbiology·Pieter-Jan CeyssensRob Lavigne
Jan 17, 2013·Environmental Microbiology·S J LabrieS W Chisholm
Nov 2, 2011·Environmental Microbiology·Hideyuki TamakiWen-Tso Liu
Feb 24, 2015·Annals of the New York Academy of Sciences·Karin Moelling, Felix Broecker
Feb 24, 2006·Virus Research·Jing LiuArcady Mushegian
Jun 13, 2015·PLoS Pathogens·Kimberley D Seed
Jun 7, 2005·Journal of Immunological Methods·Maik KlasenMatthias Wabl
Nov 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Santa S NaoremHuatao Guo
Mar 3, 2020·Frontiers in Cellular and Infection Microbiology·Yibao ChenBin Wu
Feb 14, 2020·Viruses·Aurelija M GrigonyteAndrew Millard

Related Concepts

AY029185
Metazoa
Bacteriophages
DNA Sequence
Genus Bordetella
Lysogeny
Mice, Inbred C57BL
RNA-Directed DNA Polymerase
Genetic Template
Transduction, Genetic

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.