Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge

The ISME Journal
Simon J McIlroyPer Halkjaer Nielsen

Abstract

Overgrowth of filamentous bacteria in activated sludge wastewater treatment plants (WWTPs) leads to impaired sludge settleability, a condition known as bulking, which is a common operational problem worldwide. Filaments with the Eikelboom 0092 morphotype are commonly associated with such bulking episodes. Members of the uncultured B45 phylotype, which is embraced within the phylum Chloroflexi, were recently shown to exhibit this morphology. Although these organisms are among the most abundant populations recorded in activated sludge processes, nothing is known about their metabolic characteristics. In this study, a genome sequence, representing the B45 phylotype, was retrieved from a metagenome generated from an activated sludge WWTP. The genome consisted of two chromosomes and one plasmid, which were 4.0, 1.0 and 0.04 Mbps in size, respectively. A metabolic model was constructed for this organism, based on annotation of its genome, showing its ability to generate energy by respiration, utilizing oxygen, nitrite or nitrous oxide as electron acceptors, or by fermentation of sugars. The ability of B45 members to ferment sugars under anaerobic conditions was validated in situ with microautoradiography-fluorescence in situ hybridiz...Continue Reading

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Citations

Jul 12, 2016·PloS One·Anja S ZieglerPer Halkjær Nielsen
Jul 1, 2020·International Journal of Systematic and Evolutionary Microbiology·Aharon OrenMartha E Trujillo
Jun 10, 2018·The ISME Journal·Liping HaoMorten Simonsen Dueholm
Nov 19, 2019·Frontiers in Microbiology·Andrey V MardanovAlla N Nozhevnikova
May 7, 2020·Environmental Microbiology·Maeva FinckerAlfred M Spormann
Oct 2, 2019·Frontiers in Microbiology·Lachlan B M SpeirsRobert J Seviour
Jan 6, 2021·Communications Biology·Robert NiederdorferHelmut Bürgmann
Feb 9, 2021·Frontiers in Microbiology·Patricia Bovio-WinklerClaudia Etchebehere
Jun 19, 2021·Environmental Science & Technology·Toshikazu SuenagaAkihiko Terada

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