Genomic and Phenomic Study of Mammary Pathogenic Escherichia coli

PloS One
Shlomo E BlumGabriel Leitner

Abstract

Escherichia coli is a major etiological agent of intra-mammary infections (IMI) in cows, leading to acute mastitis and causing great economic losses in dairy production worldwide. Particular strains cause persistent IMI, leading to recurrent mastitis. Virulence factors of mammary pathogenic E. coli (MPEC) involved pathogenesis of mastitis as well as those differentiating strains causing acute or persistent mastitis are largely unknown. This study aimed to identify virulence markers in MPEC through whole genome and phenome comparative analysis. MPEC strains causing acute (VL2874 and P4) or persistent (VL2732) mastitis were compared to an environmental strain (K71) and to the genomes of strains representing different E. coli pathotypes. Intra-mammary challenge in mice confirmed experimentally that the strains studied here have different pathogenic potential, and that the environmental strain K71 is non-pathogenic in the mammary gland. Analysis of whole genome sequences and predicted proteomes revealed high similarity among MPEC, whereas MPEC significantly differed from the non-mammary pathogenic strain K71, and from E. coli genomes from other pathotypes. Functional features identified in MPEC genomes and lacking in the non-mammar...Continue Reading

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Citations

Jan 17, 2016·Pathogens and Disease·Fernanda GomesMariana Henriques
Jul 21, 2016·Scientific Reports·Robert J GoldstoneDavid G E Smith
Nov 5, 2017·Applied and Environmental Microbiology·Michael A OlsonDavid L Erickson
Jan 26, 2016·PloS One·Florent KempfPierre Germon
May 10, 2017·BMC Genomics·Andreas LeimbachUlrich Dobrindt
Dec 29, 2020·Frontiers in Veterinary Science·John I AlawnehTimothy W J Olchowy
Feb 20, 2021·Microbiology Resource Announcements·Dongyun JungJennifer Ronholm
Jul 3, 2021·Proteomes·Eleni I KatsarouAngeliki I Katsafadou

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Methods Mentioned

BETA
electrophoresis
3'-phosphotransferase

Software Mentioned

megablast
ACT
snpTree
OPM package
R
ARTEMIS
CMG
PlasmidFinder
FastTree
BLAST

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