Genomic and phenotypic characterization of Escherichia coli isolates recovered from the uterus of puerperal dairy cows

Journal of Dairy Science
E SilvaL Mateus

Abstract

The role of Escherichia coli in the pathogenesis of the puerperal uterine infection of the cow is largely unknown. It is proposed that E. coli favors the persistence of Arcanobacterium pyogenes and gram-negative bacteria that are pivotal to the establishment of the infection. Here, we report the genomic and phenotypic characteristics of 72 E. coli isolates recovered from the uterus of dairy cows with normal puerperium (n = 12; 35 isolates) or clinical metritis (n = 18; 37 isolates), in an attempt to identify characteristics that are related to the establishment of uterine infection. We evaluated DNA fingerprints generated by repetitive element sequence-based PCR, phylogenetic grouping, the presence of 15 virulence factor genes, in vitro biofilm formation and its relationship to curli fimbriae expression, and cellulose production. We found a wide genetic diversity (40 clonal types), including types common to normal puerperium and clinical metritis cows (n = 6), as well as types specific to normal puerperium (n = 14) or clinical metritis (n = 20) cows. Isolates were assigned to phylogenetic groups B1 (58%), A (31%), and D (11%). Only 4 virulence factor genes were detected (hlyE, hlyA, iuc, and eaeA). In vitro biofilm formation wa...Continue Reading

References

Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J SunS Inouye
Jan 1, 1993·Transactions of the Royal Society of Tropical Medicine and Hygiene·E CupolilloH Momen
Oct 1, 1995·FEMS Immunology and Medical Microbiology·S YamamotoO Yoshida
Mar 10, 1998·Molecular Biology and Evolution·U Bergthorsson, H Ochman
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·E T RalphJ Green
Jun 26, 1999·Molecular Microbiology·J OscarssonB E Uhlin
Sep 30, 2000·Applied and Environmental Microbiology·O ClermontE Bingen
Mar 20, 2001·Infection and Immunity·U GophnaE Z Ron
Jan 17, 2002·Veterinary Microbiology·T KaipainenS Pelkonen
Mar 8, 2002·Reproduction in Domestic Animals = Zuchthygiene·L MateusJ Robalo Silva
Jun 20, 2002·Reproduction in Domestic Animals = Zuchthygiene·Luísa MateusJ Robalo Silva
May 7, 2003·Applied and Environmental Microbiology·Sandra L McLellanAlissa K Salmore
Mar 10, 2004·Applied and Environmental Microbiology·Hsiao-Hui YangBarth F Smets
Apr 13, 2004·Acta Veterinaria Scandinavica·T LehtolainenS Pelkonen
Aug 13, 2004·Journal of Veterinary Pharmacology and Therapeutics·T KutilaS Pyörälä
Jun 14, 2005·Research in Microbiology·Rob Van Houdt, Chris W Michiels
Jun 16, 2005·Journal of Clinical Microbiology·Monika KerényiTibor Pál
Nov 10, 2005·Journal of Medical Microbiology·Werner BokranzUte Römling
Jul 20, 2007·Journal of Dairy Science·G G Paulin-CurleeR Bey
Mar 28, 2008·Journal of Applied Microbiology·P NavesF Soriano
Aug 1, 2008·Animal : an International Journal of Animal Bioscience·E J WilliamsI M Sheldon
May 22, 2013·Infection and Immunity·Alexandre BleibtreuErick Denamur

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Citations

Apr 30, 2014·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Debora VogtVincent Perreten
Nov 26, 2010·Journal of Dairy Science·R C BicalhoR V V Pereira
Jan 3, 2019·Genomics & Informatics·Soo Jin Jeon, Klibs N Galvão
Sep 16, 2014·Reproduction in Domestic Animals = Zuchthygiene·S PyöräläT Katila

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