Genomic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine

Research in Microbiology
Elke De ClerckPaul De Vos

Abstract

Bacillus fumarioli was originally isolated from geothermal soils in continental and maritime Antarctica, and recently, it has been shown to be a frequent contaminant of gelatine extracts obtained from European and American production plants. These habitats are geographically widely separated, share similar temperature and pH conditions, but have substantially different organic loads. Because of the prevalence in gelatine extracts and the dissimilarity of this habitat to geothermal soil, a comparative study was performed to assess the diversity among B. fumarioli strains and reveal possible intraspecies differences that might correspond to their niches of origin. Genomic (rep-PCR, 16S rDNA sequencing, DNA-DNA hybridisations) and phenotypic techniques (analysis of fatty acid content, total cellular proteins, metabolic and morphological traits) illustrate the very close relationship between isolates from the two niches. An abundant protein band was demonstrated for gelatine isolates only. This band was shown to result from a protein with high similarity to a stress response protein. Furthermore, subtractive hybridisation revealed genomic differences between Antarctic and gelatine isolates that may indicate adaptive evolution to a ...Continue Reading

References

Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Jan 1, 1995·Analytical Biochemistry·P JenöM Horst
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·L Weiner, P Model
Mar 1, 1993·Nature Genetics·W Gish, D J States
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·N S AkopyantsD E Berg
Aug 9, 2001·International Journal of Systematic and Evolutionary Microbiology·A WillemsM Gillis
Oct 27, 2001·Applied and Environmental Microbiology·P G AgronG L Andersen
May 9, 2002·FEBS Letters·Hendrik AdamsJan Tommassen
Jun 11, 2002·International Journal of Systematic and Evolutionary Microbiology·Erko StackebrandtWilliam B Whitman
Jul 4, 2002·Molecular & Cellular Proteomics : MCP·Aaron J MackeyWilliam R Pearson
Feb 14, 2003·Systematic and Applied Microbiology·Elke De Clerck, Paul De Vos
May 7, 2003·Applied and Environmental Microbiology·Lyndsay RadnedgeGary L Andersen
May 15, 2004·International Journal of Systematic and Evolutionary Microbiology·Elke De ClerckPaul De Vos
Jun 9, 2004·Applied and Environmental Microbiology·E De ClerckP De Vos
Jul 29, 2004·International Journal of Systematic and Evolutionary Microbiology·Niall A LoganPaul De Vos

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