Genomic and phenotypic variation in epidemic-spanning Salmonella enterica serovar Enteritidis isolates.

BMC Microbiology
Laura BetancorJosé A Chabalgoity

Abstract

Salmonella enterica serovar Enteritidis (S. Enteritidis) has caused major epidemics of gastrointestinal infection in many different countries. In this study we investigate genome divergence and pathogenic potential in S. Enteritidis isolated before, during and after an epidemic in Uruguay. 266 S. Enteritidis isolates were genotyped using RAPD-PCR and a selection were subjected to PFGE analysis. From these, 29 isolates spanning different periods, genetic profiles and sources of isolation were assayed for their ability to infect human epithelial cells and subjected to comparative genomic hybridization using a Salmonella pan-array and the sequenced strain S. Enteritidis PT4 P125109 as reference. Six other isolates from distant countries were included as external comparators.Two hundred and thirty three chromosomal genes as well as the virulence plasmid were found as variable among S. Enteritidis isolates. Ten out of the 16 chromosomal regions that varied between different isolates correspond to phage-like regions. The 2 oldest pre-epidemic isolates lack phage SE20 and harbour other phage encoded genes that are absent in the sequenced strain. Besides variation in prophage, we found variation in genes involved in metabolism and bact...Continue Reading

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F C FangD G Guiney
Nov 12, 1988·Lancet·I Perales, A Audicana
Sep 1, 1988·Journal of Bacteriology·D M Roof, J R Roth
Mar 1, 1981·Journal of Bacteriology·C I Kado, S T Liu
Mar 1, 1994·The Journal of Infectious Diseases·B MishuR V Tauxe
Jan 1, 1996·Annual Review of Microbiology·J R RothT A Bobik
Aug 1, 1997·FEMS Microbiology Letters·E Allen-VercoeM J Woodward
Feb 7, 2001·Science·L R WardS J O'Brien
Sep 8, 2001·Journal of Molecular Biology·S MiroldW D Hardt
Oct 27, 2001·Applied and Environmental Microbiology·P G AgronG L Andersen
Jun 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Steffen PorwollikMichael McClelland
Jan 7, 2003·Applied and Environmental Microbiology·Burkhard MalornyReiner Helmuth
Apr 5, 2003·Journal of Applied Microbiology·T A Cogan, T J Humphrey
May 22, 2003·Advances in Experimental Medicine and Biology·Michael B PrenticeMartin J Warren
Aug 7, 2003·Applied and Environmental Microbiology·Véronique Robbe-SauleFrançoise Norel
Mar 19, 2004·Risk Analysis : an Official Publication of the Society for Risk Analysis·Tine HaldTimour Koupeev
Apr 20, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Andrew C VoetschUNKNOWN Emerging Infections Program FoodNet Working Group
May 12, 2004·Journal of Molecular Biology·Nicholas ThomsonGordon Dougan
Jan 22, 2005·Molecular Microbiology·Helen S Williamson, Andrew Free
Aug 9, 2005·Applied and Environmental Microbiology·Cesar A MoralesJean Guard-Bouldin
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hervé TettelinClaire M Fraser
Feb 8, 2006·Applied and Environmental Microbiology·Raul I ClavijoSangwei Lu
May 18, 2006·Emerging Infectious Diseases·Eleni GalanisUNKNOWN World Health Organization Global Salm-Surv
Jan 16, 2007·Science·Marie DoyleCharles J Dorman
Oct 3, 2007·BMC Microbiology·Adam B OlsonMatthew W Gilmour

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Citations

Apr 11, 2012·FEMS Microbiology Letters·Bertrand Tankouo-SandjongIsha Wallace
Jun 25, 2017·Pathogens and Disease·Claudia SilvaEdmundo Calva
Jan 1, 2012·Pathogens·Landon L WaldnerAaron P White
Nov 2, 2014·Applied and Environmental Microbiology·Andrea R McWhorterK K Chousalkar
Oct 28, 2020·Journal of Bacteriology·Aryashree ArunimaMrutyunjay Suar
Oct 28, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Fábio CampioniJuliana Pfrimer Falcão

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Datasets Mentioned

BETA
AF3353

Methods Mentioned

BETA
electrophoresis
ribotyping
genotyping
PCR

Software Mentioned

Excel
Bionumerics
Genepix Pro
GeneSpring
GraphPad Prism

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