Genomic and post-genomic effects of anti-glaucoma drugs preservatives in trabecular meshwork

Mutation Research
Alberto IzzottiSergio Claudio Saccà

Abstract

Oxidative stress plays an important role in glaucoma. Some preservatives of anti-glaucoma drugs, commonly used in glaucoma therapy, can prevent or induce oxidative stress in the trabecular meshwork. The aim of this study is to evaluate cellular and molecular damage induced in trabecular meshwork by preservatives contained in anti-glaucoma drugs. Cell viability (MTT test), DNA fragmentation (Comet test), oxidative DNA damage (8-oxo-dG), and gene expression (cDNA microarray) have been evaluated in trabecular meshwork specimens and in human trabecular meshwork cells treated with benzalkonium chloride, polyQuad, purite, and sofzia-like mixture. Moreover, antimicrobial effectiveness and safety of preservative contents in drugs was tested. In ex vivo experiments, benzalkonium chloride and polyQuad induced high level of DNA damage in trabecular meshwork specimens, while the effect of purite and sofzia were more attenuated. The level of DNA fragmentation induced by benzalkonium chloride was 2.4-fold higher in subjects older than 50 years than in younger subjects. Benzalkonium chloride, and polyQuad significantly increased oxidative DNA damage as compared to sham-treated specimens. Gene expression was altered by benzalkonium chloride, p...Continue Reading

References

Nov 1, 1990·Ophthalmology·T L YoungM D Farber
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Apr 28, 2004·Investigative Ophthalmology & Visual Science·Pierre-Jean PisellaChristophe Baudouin
Feb 6, 2007·Advances in Therapy·Mitchell H FriedlaenderMichelle Senchyna
Oct 31, 2008·Investigative Ophthalmology & Visual Science·Alice L YuUlrich Welge-Lüssen
Jun 12, 2009·Investigative Ophthalmology & Visual Science·Alberto IzzottiCristina Cartiglia
Jun 16, 2010·Archives of Ophthalmology·Alberto IzzottiCristina Cartiglia
Jul 30, 2010·Journal of Proteome Research·A IzzottiS C Saccà
Aug 5, 2010·Mutation Research·José Manuel Pérez MartínMaría José Hazen

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Citations

Feb 28, 2020·Oxidative Medicine and Cellular Longevity·Anton G RogovRenata A Zvyagilskaya
Dec 8, 2020·Expert Opinion on Drug Delivery·Michele FigusChiara Posarelli
Feb 13, 2021·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ejaz Ansari
Jul 16, 2021·Eye·Michael H GoldsteinSrilatha Vantipalli
Oct 20, 2020·Progress in Retinal and Eye Research·Christophe BaudouinElisabeth M Messmer

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