Genomic and probiotic characterization of SJP-SNU strain of Pichia kudriavzevii

AMB Express
Seung-Min HongJae-Hong Kim

Abstract

The yeast strain SJP-SNU was investigated as a probiotic and was characterized with respect to growth temperature, bile salt resistance, hydrogen sulfide reducing activity, intestinal survival ability and chicken embryo pathogenicity. In addition, we determined the complete genomic and mitochondrial sequences of SJP-SNU and conducted comparative genomics analyses. SJP-SNU grew rapidly at 37 °C and formed colonies on MacConkey agar containing bile salt. SJP-SNU reduced hydrogen sulfide produced by Salmonella serotype Enteritidis and, after being fed to 4-week-old chickens, could be isolated from cecal feces. SJP-SNU did not cause mortality in 10-day-old chicken embryos. From 13 initial contigs, 11 were finally assembled and represented 10 chromosomal sequences and 1 mitochondrial DNA sequence. Comparative genomic analyses revealed that SJP-SNU was a strain of Pichia kudriavzevii. Although SJP-SNU possesses pathogenicity-related genes, they showed very low amino acid sequence identities to those of Candida albicans. Furthermore, SJP-SNU possessed useful genes, such as phytases and cellulase. Thus, SJP-SNU is a useful yeast possessing the basic traits of a probiotic, and further studies to demonstrate its efficacy as a probiotic i...Continue Reading

References

Jan 1, 1976·Antonie van Leeuwenhoek·C P Kurtzman, M J Smiley
Mar 1, 1971·The Journal of Applied Bacteriology·T Wainwright
Dec 1, 1993·International Journal of Food Microbiology·D H Oh, D L Marshall
Jul 1, 1996·Journal of Clinical Microbiology·A CarlottiJ Villard
Sep 17, 1999·International Journal of Food Microbiology·T R Klaenhammer, M J Kullen
Oct 9, 1999·Journal of Bacteriology·G MarinoniJ Piskur
Mar 16, 2001·FEMS Microbiology Reviews·F Navarro-GarcíaJ Pla
Mar 28, 2002·Applied and Environmental Microbiology·Covadonga R AriasMickey E Parish
Dec 3, 2002·Science·Duncan GreigMichael Travisano
May 15, 2003·International Journal of Food Microbiology·J K MugulaT Sørhaug
Dec 9, 2003·Applied and Environmental Microbiology·Christiane B MerothChristian Hertel
Mar 12, 2004·Molecular Genetics and Genomics : MGG·Z GojkovićJ Piskur
May 28, 2004·Journal of Biotechnology·Anne Casey, Gary Walsh
Jun 25, 2004·Nucleic Acids Research·Mario StankeBurkhard Morgenstern
Dec 6, 2006·Drug Metabolism Reviews·Don H TruongPeter J O'Brien
Dec 29, 2006·Yeast·I S Ishtar Snoek, H Yde Steensma
Feb 28, 2007·Antimicrobial Agents and Chemotherapy·Jennifer Nielsen KahnDavid S Perlin
Mar 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Isheng J TsaiVassiliki Koufopanou
May 26, 2009·Nature·Geraldine ButlerChristina A Cuomo
Apr 28, 2010·Infection and Immunity·Ilse D JacobsenMatthias Brock
Jun 1, 2010·Food Microbiology·K ZottI Masneuf-Pomarede
Jun 19, 2010·Nature Reviews. Genetics·Bernard Dujon
Sep 14, 2011·Enzyme Research·Ramesh Chander KuhadAjay Singh
Mar 17, 2012·Therapeutic Advances in Gastroenterology·Theodoros Kelesidis, Charalabos Pothoulakis
Oct 3, 2012·Eukaryotic Cell·Giek Far ChanNoor Aini Abdul Rashid
Apr 20, 2014·Applied Microbiology and Biotechnology·Mudassar AhmadHelmut Schwab

❮ Previous
Next ❯

Citations

May 14, 2020·Medical Mycology·A T JamiuC H Pohl
Mar 31, 2020·International Journal of Food Microbiology·Raffaella Di CagnoMarco Gobbetti

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA383123

Software Mentioned

BioEdit
RepeatMasker
AUGUSTUS
GARM metaassembler
SSR Finder
FALCON
INFERNAL Tool
RepeatModeler
BLAST
HGAP3

Related Concepts

Related Feeds

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.