Abstract
Examining global effects of toxic metals on gene expression can be useful for elucidating patterns of biological response, discovering underlying mechanisms of toxicity, and identifying candidate metal-specific genetic markers of exposure and response. Using a 1,200 gene nylon array, we examined changes in gene expression following low-dose, acute exposures of cadmium, chromium, arsenic, nickel, or mitomycin C (MMC) in BEAS-2B human bronchial epithelial cells. Total RNA was isolated from cells exposed to 3 M Cd(II) (as cadmium chloride), 10 M Cr(VI) (as sodium dichromate), 3 g/cm2 Ni(II) (as nickel subsulfide), 5 M or 50 M As(III) (as sodium arsenite), or 1 M MMC for 4 hr. Expression changes were verified at the protein level for several genes. Only a small subset of genes was differentially expressed in response to each agent: Cd, Cr, Ni, As (5 M), As (50 M), and MMC each differentially altered the expression of 25, 44, 31, 110, 65, and 16 individual genes, respectively. Few genes were commonly expressed among the various treatments. Only one gene was altered in response to all four metals (hsp90), and no gene overlapped among all five treatments. We also compared low-dose (5 M, noncytotoxic) and high-dose (50 M, cyt...Continue Reading
References
Jan 1, 1990·International Review of Experimental Pathology·J A Alcedo, K E Wetterhahn
Jan 1, 1989·Molecular Carcinogenesis·J W Hamilton, K E Wetterhahn
May 1, 1989·Environmental Health Perspectives·D A Edelman, V L Roggli
Mar 13, 1989·FEBS Letters·S TaketaniR Tokunaga
Jul 1, 1971·The Journal of Cell Biology·R Ross
Jan 1, 1980·Mutation Research·A Léonard, R R Lauwerys
Aug 1, 1994·Molecular Carcinogenesis·J McCaffreyJ W Hamilton
Jan 1, 1996·International Archives of Occupational and Environmental Health·D M ByrdS Lai
Jan 1, 1996·Free Radical Biology & Medicine·Y LiuN J Holbrook
Feb 1, 1997·Carcinogenesis·A HartwigL H Mullenders
Jun 1, 1997·Toxicology and Applied Pharmacology·D Beyersmann, S Hechtenberg
Aug 1, 1997·Regulatory Toxicology and Pharmacology : RTP·J Barnhart
Dec 24, 1997·Environmental Health Perspectives·A BarchowskyM D Treadwell
Aug 14, 1998·Environmental Health Perspectives·J W HamiltonJ P Lariviere
Oct 24, 1998·Biochemical and Biophysical Research Communications·D B MenzelR N Roth
Feb 18, 1999·Toxicology Letters·A Hartwig
Jun 24, 1999·Environmental Health Perspectives·C O AbernathyM Waalkes
Jun 26, 1999·Journal of Toxicology. Clinical Toxicology·D G Barceloux
Jan 5, 2000·Oncogene·A C PorterR R Vaillancourt
Apr 27, 2000·Toxicology·T Gebel
Sep 23, 2000·Toxicology and Applied Pharmacology·A Andrew, A Barchowsky
Oct 12, 2000·Molecular and Cellular Biology·S A JelinskyL D Samson
Oct 26, 2000·Environmental Health Perspectives·F LadenJ Schwartz
Feb 15, 2001·Environmental Health Perspectives·M BartosiewiczA Buckpitt
Feb 28, 2001·Cancer Treatment and Research·M D Williams, A B Sandler
Mar 31, 2001·International Journal of Hygiene and Environmental Health·T W Gebel
May 16, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·J LiuM P Waalkes
Jun 19, 2001·Mutation Research·B L Vogt, T G Rossman
Aug 16, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·A S AndrewA Barchowsky
Oct 2, 2001·Toxicology and Applied Pharmacology·T G RossmanM C Bosland
Oct 5, 2001·Free Radical Biology & Medicine·E MinetC Michiels
Jan 5, 2002·Human & Experimental Toxicology·A D Dayan, A J Paine
Mar 30, 2002·Critical Reviews in Oncology/hematology·E Denkhaus, K Salnikow
May 16, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hisham K HamadehCynthia A Afshari
May 16, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hisham K HamadehCynthia A Afshari
May 23, 2002·Carcinogenesis·Ling-Huei YihTe-Chang Lee
Aug 27, 2002·Environmental Health Perspectives·George D Leikauf
Sep 21, 2002·Cell Biology and Toxicology·C BaudouinY Gall
Feb 6, 2003·International Journal of Cancer. Journal International Du Cancer·Angeline S AndrewJoshua W Hamilton
Mar 14, 2003·Environmental Health Perspectives·Dong-Soon BaeJulie A Campain
Citations
Sep 4, 2004·Environmental Health Perspectives·Guido SteinerStefan Ruepp
May 17, 2005·Molecular Pharmacology·Simone KannAlvaro Puga
Nov 3, 2006·The Biochemical Journal·Kimberley A O'HaraAaron Barchowsky
Sep 13, 2006·Environmental Health Perspectives·Samuel C McNeelyJ Christopher States
Nov 21, 2008·International Archives of Occupational and Environmental Health·Dirk TaegerBeate Pesch
Jul 9, 2009·Molecular Biology Reports·Lei ZhangGuanpin Yang
Jul 18, 2009·Phytochemical Analysis : PCA·Marina M A CamposCarlos E S Miranda
Mar 26, 2011·PloS One·Hong SunMax Costa
Jul 29, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·H J ClewellJ W Yager
Sep 15, 2011·Ecotoxicology·Zi-Cheng LiLin-Bo Zhang
Oct 7, 2011·International Journal of Phytoremediation·Alka KumariPiar Chand
Apr 3, 2012·The Journal of Biological Chemistry·Jennifer M BombergerBruce A Stanton
Feb 5, 2013·International Journal of Molecular Sciences·Fairuz Liyana Mohd RasdiSharifah Mohamad
Jul 6, 2013·Oncology Reports·Jee Young KwonYoung Rok Seo
Nov 2, 2016·Proteomics. Clinical Applications·Yan-Ming XuAndy T Y Lau
Jul 6, 2016·Fish Physiology and Biochemistry·Sangeeta DeySuchismita Das
Feb 25, 2017·Food & Function·Yingying HuangZhongyi Yang
Feb 23, 2020·Integrated Environmental Assessment and Management·Daniel Gomes CoelhoJuraci Alves de Oliveira
Jan 27, 2005·Proteomics·Odette PratEric Quemeneur
Oct 7, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ju-Pi LiJia-Ling Yang
Jan 5, 2005·Molecular Pharmacology·Jessica A BonzoRobert H Tukey
Dec 7, 2005·Environmental Health Perspectives·Zhuowei LiYuh-Chin T Huang
Oct 31, 2008·Proteomics·Ingrid MillerElisabetta Gianazza
Feb 22, 2008·Environmental Health Perspectives·Jennifer C DaveyJoshua W Hamilton
Aug 3, 2006·Physiological Genomics·Siew Hong LamZhiyuan Gong
Sep 15, 2011·Fish Physiology and Biochemistry·Haichao LiuXufa Ma
Dec 18, 2010·Bulletin of Environmental Contamination and Toxicology·Yogesh B PakadeDhananjay Kumar Tewary
Jun 11, 2009·Journal of Clinical Immunology·Nilanjana BanerjeeAshok K Giri
Mar 26, 2010·Molecular Carcinogenesis·André N CostaM Carmen Alpoim
Aug 8, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Angeline S AndrewJoshua W Hamilton
Jun 25, 2009·Environmental and Molecular Mutagenesis·P Robinan GentryHarvey J Clewell
Apr 22, 2009·Molecular Nutrition & Food Research·Maike NollenTanja Schwerdtle
Sep 23, 2014·Oxidative Medicine and Cellular Longevity·Jae Sung ParkYoung Rok Seo
Jan 14, 2016·Oncotarget·Soyoung ParkDazhong Xu
May 5, 2017·Carcinogenesis·Susan E ScanlonPeter M Glazer
Feb 10, 2018·Journal of Applied Toxicology : JAT·Zhan-Ling LiangAndy T Y Lau
Apr 25, 2018·Nanotoxicology·Joseph D T NdikaAnne Puustinen
Aug 9, 2019·Cells·Heng Wee TanAndy T Y Lau
Jan 10, 2004·Toxicologic Pathology·Xing CuiSeishiro Hirano
Dec 16, 2004·Environmental Health Perspectives·Magali TaulanMarie-Catherine Romey
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anthony LemarieLaurent Vernhet
Oct 31, 2008·Environmental Health : a Global Access Science Source·Jean-Paul BourdineaudMuriel Laclau
Apr 17, 2008·Environmental Health Perspectives·Angeline S AndrewMargaret R Karagas
Sep 7, 2007·Environmental Health Perspectives·Herbert RiechelmannRudolf Reiter
Apr 5, 2011·BMC Genomics·Margaret Ann BentonRebecca C Fry
Jun 2, 2012·American Journal of Respiratory and Critical Care Medicine·Stefanie N LinchJeffrey A Gold
Jan 22, 2015·Journal of Exposure Science & Environmental Epidemiology·Margaret Kurzius-SpencerJefferey L Burgess
Jun 1, 2012·Analytical and Bioanalytical Chemistry·Hongying ZhongLing Dai
Aug 6, 2009·Environmental Health Perspectives·Courtney D KozulJoshua W Hamilton