Genomic array and expression analysis of frequent high-level amplifications in adenocarcinomas of the gastro-esophageal junction

Cancer Genetics and Cytogenetics
Herman van DekkenKaroly Szuhai

Abstract

Adenocarcinomas of the gastroesophageal junction (GEJ) show frequent high-level amplifications (HLA), but the underlying genes are not well defined. We have characterized genomic gain in 14 GEJ carcinomas by array-based comparative genomic hybridization (aCGH). The most frequent gains and amplifications were detected at 7q (57%), 8q (57%), 17q (64%), and 20q (79%), with minimally amplified regions at 7q21.1, 8q24.2, 17q12, and 20q13.2. Five HLA were detected on 7q, one on 8q, two on 17q, and three on 20q. HLA of 8q24 and 17q12 were related to MYC and ERBB2, respectively. The HLA on 7q21 was associated recurrently with ABCB1, whereas the amplified region on 20q13 implicated ZNF217, BCAS1, and CYP24. RNA expression analysis of 11 adenocarcinomas by reverse-transcription polymerase chain reaction was performed for cancer-related genes residing at 7q21 (ABCB1, ABCB4, CDK6, HGF, DMTF1, SRI, TP53AP1) and 20q13 (ZNF217, BCAS1, CYP24, TNFRSF6B). The most frequently upregulated gene on 7q21 was HGF (45%), but there was no association with genomic amplification. The most frequently overexpressed gene at 20q13 was BCAS1 (27%), which was related to HLA of this region (P = 0.006) in all three cases. We conclude that HLA occur often in GEJ a...Continue Reading

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Citations

Jul 28, 2011·JAMA : the Journal of the American Medical Association·Mohammed OrloffCharis Eng
Jan 21, 2012·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Zhengyu FangJun Wan
Sep 30, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Giulia PaselloDaniela Saggioro
May 7, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Thomas G PaulsonBrian J Reid
May 20, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Amin IsmailTony E Godfrey
Sep 13, 2012·World Journal of Gastroenterology : WJG·Yuan-Yuan LiuJi-Yun Lee
Mar 16, 2018·International Journal of Molecular Sciences·David VranaPavel Soucek
May 22, 2008·British Journal of Cancer·J K R BoultC Tselepis
Dec 21, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Guoan ChenNithya Ramnath
Apr 12, 2013·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Alok Kishorkumar ShahMichelle M Hill

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