Genomic but not antigenomic hepatitis delta virus RNA is preferentially exported from the nucleus immediately after synthesis and processing

Journal of Virology
T B Macnaughton, Michael M C Lai

Abstract

Hepatitis delta virus (HDV) contains a viroid-like circular RNA that replicates via a double rolling circle replication mechanism. It is generally assumed that HDV RNA is synthesized and remains exclusively in the nucleus until being exported to the cytoplasm for virion assembly. Using a [32P]orthophosphate metabolic labeling procedure to study HDV RNA replication (T. B. Macnaughton, S. T. Shi, L. E. Modahl, and M. M. C. Lai. J. Virol. 76:3920-3927, 2002), we unexpectedly found that a significant amount of newly synthesized HDV RNA was detected in the cytoplasm. Surprisingly, Northern blot analysis revealed that the genomic-sense HDV RNA is present almost equally in both the nucleus and cytoplasm, whereas antigenomic HDV RNA was mostly retained in the nucleus, suggesting the specific and highly selective export of genomic HDV RNA. Kinetic studies showed that genomic HDV RNA was exported soon after synthesis. However, only the monomer and, to a lesser extent, the dimer HDV RNAs were exported to the cytoplasm; very little higher-molecular-weight HDV RNA species were detected in the cytoplasm. These results suggest that the cleavage and processing of HDV RNA may facilitate RNA export. The export of genomic HDV RNA was resistant to...Continue Reading

References

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Citations

Apr 23, 2003·Trends in Microbiology·John M Taylor
Nov 23, 2007·Journal of Virology·Jinhong ChangJohn Taylor
Aug 14, 2003·Journal of Virology·Jinhong Chang, John M Taylor
Oct 29, 2003·Journal of Virology·Jinhong ChangJohn M Taylor
Mar 16, 2006·Journal of Virology·Jinhong ChangJohn Taylor
Oct 12, 2012·Bioinformation·Sarita SinghPrahlad Kishore Seth
Oct 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Swee Kee Wong, David W Lazinski
Feb 17, 2015·Pathogens·Rafael AldabeGloria González-Aseguinolaza
Jan 4, 2006·Molecular and Cellular Endocrinology·Sanjay KumarRakesh K Tyagi
Apr 17, 2016·Journal of Hepatology·Camille Sureau, Francesco Negro
Jun 15, 2004·Virology·Senthil K RadhakrishnanAndrei L Gartel
Dec 21, 2005·Virology·John M Taylor
Aug 19, 2016·Nature Reviews. Gastroenterology & Hepatology·Florian A LemppStephan Urban
Dec 1, 2009·Viruses·Chung-Hsin Tseng, Michael M C Lai
Nov 4, 2016·The Journal of Biological Chemistry·Hsiu-Chen HuangCheng Huang
Sep 6, 2018·Cold Spring Harbor Protocols·James DeCaprio, Thomas O Kohl
Jul 3, 2021·Viruses·Jimena Pérez-VargasNatalia Freitas
Jun 14, 2019·Journal of Advanced Research·Nathalie MenthaDulce Alfaiate

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