Genomic changes arising in long-term stab cultures of Escherichia coli

Journal of Bacteriology
D FaureJ Adams

Abstract

Genomic scans of clones isolated from long-term stab cultures of Escherichia coli K-12 showed the loss of two large segments of the genome, with each lost segment being approximately 20 kb long. A detailed analysis of one of the deletions, located between 5.4 and 5.9 min, revealed that similar deletions had arisen in several other stab cultures. All deletions of this type exhibited a right terminus ending precisely at an IS5A element and a left terminus that varied over an approximately 5-kb range but was bordered in all but two cases by sequences belonging to the preferred consensus target sequence for IS5, YTAR. The ubiquity of such deletions in independent stab cultures and the increase in their frequency over time argue that they have a selective advantage. It is speculated that the loss of the crl locus is responsible for the selective advantage of the deletions.

References

Jan 1, 1977·Annual Review of Microbiology·R P Anderson, J R Roth
Jan 1, 1975·Molecular & General Genetics : MGG·H J Reif, H Saedler
Jan 1, 1985·Molecular & General Genetics : MGG·A P Jessop, C Clugston
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Jan 1, 1983·Progress in Nucleic Acid Research and Molecular Biology·W Arber
Sep 5, 1997·Science·F R BlattnerY Shao
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·J Mahillon, M Chandler
Oct 10, 1998·Molecular Microbiology·L A Pratt, T J Silhavy
May 14, 1999·Current Opinion in Microbiology·T Ferenci
Jul 27, 2000·Journal of Bacteriology·A SuttonA Eisenstark
Jan 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·M M RiehleA D Long
May 30, 2001·FEMS Microbiology Letters·K EdwardsA Eisenstark
Oct 5, 2001·Trends in Genetics : TIG·A MiraN A Moran
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Bethany YeiserSteven E Finkel
Nov 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Maitreya J DunhamDavid Botstein
Mar 27, 2003·Molecular Microbiology·Thomas Nyström
Oct 24, 2003·Current Opinion in Microbiology·Nancy A Moran
Mar 5, 2004·Journal of Bacteriology·Steffen PorwollikMichael McClelland
Jan 1, 1983·Evolution; International Journal of Organic Evolution·Lin Chao, Edward C Cox

❮ Previous
Next ❯

Citations

Jan 18, 2006·Nature Reviews. Microbiology·Steven E Finkel
Feb 13, 2007·Journal of Bacteriology·Véronique Robbe-SauleFrançoise Norel
Nov 15, 2011·Journal of Bacteriology·Peter L FreddolinoSaeed Tavazoie
Jul 31, 2013·Environmental Microbiology·Gustavo EydallinThomas Ferenci
Nov 28, 2006·Metabolic Engineering·Shamik S SharmaSarah W Harcum
Sep 17, 2008·FEMS Microbiology Letters·Ranjna MadanSubramony Mahadevan
Feb 20, 2018·Environmental Microbiology·Annie I Chen, Mark Goulian
Jan 21, 2020·Microbiology·Matthew J Dorman, Nicholas R Thomson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.