Genomic characterization and comparison of seven Myoviridae bacteriophage infecting Bacillus thuringiensis

Virology
Amber Brooke SauderLouise Temple

Abstract

Bacillus thuringiensis Kurstaki, a bacterium that is a source of biopesticides and a safe simulant for pathogenic Bacillus species, was used to isolate seven unique bacteriophages. The phage genomes were sequenced and ranged in size from 158,100 to 163,019 bp encoding 290-299 genes, and the GC content of ~38% was similar to that of the host bacterium. All phages had terminal repeats 2-3 kb long. Three of the phages encoded tRNAs and three contained a self-splicing intron in the DNA polymerase gene. They were categorized as a single cluster (>60% nucleotide conservation) containing three subclusters (>80% nucleotide conservation), supported by genomic synteny and phylogenetic analysis. Considering the published genomes of phages that infect the genus Bacillus and noting the ability of many of the Bacillus cereus group phages to infect multiple species, a clustering system based on gene content is proposed.

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Citations

Aug 19, 2016·Frontiers in Microbiology·Callum J CooperAnders S Nilsson
Aug 20, 2016·Genome Announcements·Jessica L CornellLouise Temple
Oct 1, 2016·Genome Announcements·Ivan ErillUNKNOWN 2015 UMBC Phage Hunters
Oct 16, 2016·Genome Announcements·Eileen F BreslinLouise Temple
Mar 8, 2018·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Allan Ayella, Moriah R Beck
Aug 5, 2017·Genome Announcements·Ivan ErillUNKNOWN 2016 UMBC Phage Hunters
Mar 9, 2019·Archives of Virology·Birthe NordmannRobert Hertel
Dec 14, 2019·Microbiology Resource Announcements·Yoga A HandokoJulianne H Grose
Mar 11, 2017·Genome Announcements·Kelly FlounlackerUNKNOWN the 2015–2016 VCU Phage Hunters
Dec 12, 2018·Microbiology Resource Announcements·Elizabeth GreguskeKarolina Fucikova
May 12, 2018·Genome Announcements·Brenna KentUNKNOWN the 2016–2017 VCU Phage Hunters
Apr 13, 2019·Microbiology Resource Announcements·Ryan ShowalterLouise Temple
Mar 19, 2020·Frontiers in Medicine·Rhea LewisColin Hill
Dec 24, 2018·Microbiology Resource Announcements·Elizabeth Greguske
Apr 18, 2020·Microbiology Resource Announcements·Trever L ThurgoodJulianne H Grose
Jan 9, 2021·Microbiology Resource Announcements·Julie DuperierElizabeth Greguske
May 6, 2021·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Muhammad AsifShafiq-Ur- Rehman

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