Genomic Characterization of Listeria monocytogenes Isolated From Ready-to-Eat Meat and Meat Processing Environments in Poland

Frontiers in Microbiology
Monika KurpasKinga Wieczorek

Abstract

Listeria monocytogenes is one of the major foodborne pathogens. Isolates of PCR-serogroups IIb (n = 17) and IVb (n = 31) recovered from food (n = 33) and food processing environment (n = 15) in Poland were characterized using whole genome sequencing. Most isolates belonged to Multi-Locus Sequence Type (MLST) ST2 (31.3%) and ST5 (22.9%). Core genome MLST (cgMLST) analysis classified isolates into seven sublineages (SL) and 25 different cgMLST types (CT). Consistent with the MLST results, most sublineages were SL2 and SL5. Eleven isolates harbored aacA4 encoding resistance to aminoglycosides, three isolates harbored emrC (n = 3) and one brcABC (n = 1) encoding tolerance to benzalkonium chloride. Isolates belonging to SL5 CT2323 carried a so far unreported inlB allele with a deletion of 141 nucleotides encoding the β-repeat sheet and partially the GW1 domain of InlB. Comparison with publicly available genome sequences from L. monocytogenes isolated from human listeriosis cases in Poland from 2004 to 2013 revealed five common CTs, suggesting a possible epidemiological link with these strains. The present study contributes to characterize the diversity of L. monocytogenes in ready-to-eat (RTE) meat and meat processing environments i...Continue Reading

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Citations

Jul 27, 2021·Critical Reviews in Food Science and Nutrition·Jiali JiMonique Lacroix
Aug 20, 2021·International Journal of Food Microbiology·Francesca MaggioAntonello Paparella
Oct 15, 2021·Archives of Microbiology·Jucilene Sena Dos SantosLuciana Ruschel Dos Santos

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Datasets Mentioned

BETA
PRJNA629756

Software Mentioned

Lm
cgMLST
iTOL
MEGA7
BIGSdb
SPAdes
Bionumerics
BLAST
fqCleaner

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