Genomic characterization of lung flukes, Paragonimus heterotremus, P. siamensis, P. harinasutai, P. westermani and P. bangkokensis by RAPD markers

Veterinary Parasitology
Pewpan M IntapanW Maleewong

Abstract

Random amplified polymorphic DNA (RAPD) markers were assayed in an attempt to discriminate among five species of Paragonimus. Genomic DNAs of two strains of Paragonimus heterotremus from two provinces in Thailand, Saraburi and Phitsanulok, as well as of P. siamensis, P. harinasutai, P. westermani and P. bangkokensis were extracted and amplified by an arbitrary primer, namely P2 (5-GTTTCGCTCC-3). RAPD patterns showed that those five species were genetically distinct, although they shared genomic DNA to some extent. This primer could also distinguish between two strains of P. heterotremus. The polymorphism observed allowed to construct a relationship dendrogram. The phylogenetic dendrogram showed that the P. heterotremus strains were closest to P. harinasutai, followed by P. siamensis, P. bangkokensis and P. westermani.

References

Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Dec 25, 1990·Nucleic Acids Research·J Welsh, M McClelland
Nov 1, 1993·Transactions of the Royal Society of Tropical Medicine and Hygiene·U M MorganR C Thompson
Dec 1, 1999·International Journal for Parasitology·L van HerwerdenT Agatsuma
Nov 7, 2001·The Journal of Parasitology·P M Intapan, W Maleewong

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Citations

Dec 19, 2012·Parasitology International·Chairat TantrawatpanWanchai Maleewong
Jul 31, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Chairat TantrawatpanWanchai Maleewong

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