Genomic characterization of mycobacteriophage Giles: evidence for phage acquisition of host DNA by illegitimate recombination

Journal of Bacteriology
Peter MorrisGraham F Hatfull

Abstract

A characteristic feature of bacteriophage genomes is that they are architecturally mosaic, with each individual genome representing a unique assemblage of individual exchangeable modules. Plausible mechanisms for generating mosaicism include homologous recombination at shared boundary sequences of module junctions, illegitimate recombination in a non-sequence-directed process, and site-specific recombination. Analysis of the novel mycobacteriophage Giles genome not only extends our current perspective on bacteriophage genetic diversity, with more than 60% of the genes unrelated to other mycobacteriophages, but offers novel insights into how mosaic genomes are created. In one example, the integration/excision cassette is atypically situated within the structural gene operon and could have moved there either by illegitimate recombination or more plausibly via integrase-mediated site-specific recombination. In a second example, a DNA segment has been recently acquired from the host bacterial chromosome by illegitimate recombination, providing further evidence that phage genomic mosaicism is generated by nontargeted recombination processes.

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Citations

Mar 2, 2010·Applied Microbiology and Biotechnology·Matthias Brigulla, Wilfried Wackernagel
Oct 17, 2008·Nature Reviews. Microbiology·Julia C van KesselGraham F Hatfull
Jul 16, 2013·Applied and Environmental Microbiology·Mariana PiuriGraham F Hatfull
Sep 12, 2012·Journal of Bacteriology·Lamia OulmiVéronique Roux
Feb 24, 2012·Journal of Virology·Catherine MageeneyGraham F Hatfull
Jun 10, 2010·Annual Review of Microbiology·Graham F Hatfull
Nov 3, 2014·Annual Review of Virology·Graham F Hatfull, Vincent Racaniello
Jul 26, 2008·Research in Microbiology·Graham F HatfullRoger W Hendrix
Aug 8, 2009·Environmental Microbiology·Florent AnglyForest Rohwer
Jun 27, 2009·Molecular Microbiology·Kimberly PayneGraham F Hatfull
May 31, 2014·Journal of Microbiological Methods·Feng YangTianyu Zhang
Jun 6, 2012·Bacteriophage·Laura J MarinelliMariana Piuri
Jul 3, 2013·Bacteriophage·Gregory W Broussard, Graham F Hatfull
Oct 1, 2008·Current Opinion in Microbiology·Graham F Hatfull
Aug 10, 2016·The ISME Journal·Rohit GhaiFrancisco Rodriguez-Valera
Dec 1, 2017·Scientific Reports·Jitender MehlaPeter Uetz
May 20, 2015·Journal of Bacteriology·Jitender MehlaPeter Uetz
Feb 28, 2019·PloS One·Cristian Alejandro SuarezHéctor Ricardo Morbidoni
Sep 30, 2020·Annual Review of Virology·Graham F Hatfull
Dec 13, 2018·MBio·Kenan C MurphyChristopher M Sassetti
Dec 7, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Zhen GongJianping Xie
Jan 16, 2021·Microbiology Resource Announcements·Elizabeth D AmarhGraham F Hatfull
Dec 22, 2020·The ISME Journal·Anna DragošÁkos T Kovács

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