Genomic Characterization of Picornaviruses Isolated From Ribbon (Histriophoca fasciata ) and Harbor (Phoca vitulina ) Seals

Frontiers in Veterinary Science
Thais C S RodriguesThomas B Waltzek

Abstract

The seal picornavirus 1, species Aquamavirus A, is currently the only recognized member of the genus Aquamavirus within the family Picornaviridae. The bear picornavirus 1 was recently proposed as the second species in the genus under the name aquamavirus B. Herein, we determined the complete genomes of two novel pinniped picornaviruses, the harbor seal picornavirus (HsPV) and the ribbon seal picornavirus (RsPV). The HsPV and the RsPV were isolated in Vero.DogSLAMtag cells from samples collected from stranded harbor (Phoca vitulina) and ribbon (Histriophoca fasciata) seals. RsPV-infected Vero.DogSLAMtag cells displaying extensive cytopathic effects were processed for transmission electron microscopy and revealed non-enveloped viral particles aggregated into paracrystalline arrays in the cytoplasm. A next-generation sequencing approach was used to recover the complete genomes of the HsPV and the RsPV (6,709 and 6,683 bp, respectively). Phylogenetic and genetic analyses supported the HsPV and the RsPV as members of the Aquamavirus genus. Based on these results, RsPV represents a novel strain of Aquamavirus A, while the HsPV is a novel strain of the proposed species aquamavirus B. These discoveries provide information on the evolut...Continue Reading

References

Jan 9, 2001·Clinical Microbiology Reviews·J A Cuthbert
Mar 20, 2001·Veterinary Research·F SobrinoV Ley
May 21, 2004·Nature Reviews. Microbiology·Philip D Minor
Oct 6, 2005·Nature Reviews. Microbiology·J Lindsay WhittonRalph Feuer
Oct 19, 2007·Journal of Virology·A KapoorE Delwart
Apr 17, 2008·Nature Reviews. Microbiology·Sven Miller, Jacomine Krijnse-Locker
Dec 1, 1994·Avian Pathology : Journal of the W.V.P.A·G A Tannock, D R Shafren
Apr 1, 2009·Emerging Infectious Diseases·M Steven ObersteTadeusz J Kochel
Jul 8, 2009·Veterinary Microbiology·Hendrik H NollensStephanie Venn-Watson
Nov 27, 2009·Medical Microbiology and Immunology·Holger F RabenauHans Wilhelm Doerr
Apr 17, 2010·Current Topics in Microbiology and Immunology·Tobias J TuthillDavid J Rowlands
Jul 29, 2011·Journal of Virology·Linlin LiEric Delwart
Jul 29, 2011·The Journal of General Virology·Gustavo PalaciosW Ian Lipkin
Jan 9, 2013·Clinical Microbiology Reviews·Samantha E JacobsThomas J Walsh
Sep 27, 2014·PloS One·Brejnev Muhizi MuhireDarren Patrick Martin
Jul 15, 2015·Molecular Reproduction and Development·Johannes SchindelinKevin W Eliceiri
Sep 9, 2017·The Journal of General Virology·R Zell Ictv Report Consortium
Dec 21, 2018·Archives of Virology·Haoning WangTongling Shan
May 19, 2020·The Journal of General Virology·Elizabeth C ScherbatskoyThomas B Waltzek

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Datasets Mentioned

BETA
EU142040

Methods Mentioned

BETA
PCR

Software Mentioned

Sequence Demarcation Tool
IQ
BLASTX
Geneious Prime
CLC Genomics Workbench
ImageJ2

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