Genomic characterization of POS5, the Saccharomyces cerevisiae mitochondrial NADH kinase

Mitochondrion
Kevin V ShiannaMicheline K Strand

Abstract

Disruption of the Saccharomyces cerevisiae mitochondrial NADH kinase POS5 increases the mitochondrial mutation rate 50-fold. Whereas most multicellular eukaryotic genomes have one NADH kinase gene, the yeast genome contains three distinct genes encoding NAD/H kinase activity. To determine if all three genes are essential for viability we constructed combinations of gene knockouts. We show that only the pos5Deltautr1Delta combination is synthetically lethal, demonstrating an essential overlapping function, and showing that NAD/H kinase activity is essential for eukaryotic viability. The single human NAD/H kinase gene can rescue the lethality of the double knockout in yeast, demonstrating that the single human gene can fill the various functions provided by the three yeast genes. The human NAD/H kinase gene harbors very common sequence variants, but all of these equally complement the synthetic lethality in yeast, illustrating that each of these are functionally wild-type. To understand the molecular mechanism of the mitochondrial genome instability of pos5 mutation we performed gene expression analysis on the pos5Delta. The pos5Delta resulted in an increase in expression of most of the iron transport genes including key genes in...Continue Reading

References

Jan 1, 1985·The International Journal of Biochemistry·E T McGuinness, J R Butler
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H N Kirkman, G F Gaetani
Jan 1, 1995·Methods in Enzymology·A Holmgren, M Björnstedt
Apr 1, 1997·The American Journal of Physiology·A L NieminenJ J Lemasters
Aug 18, 1997·Biochemical and Biophysical Research Communications·T TamuraT N Hansen
Mar 5, 1999·Science·D C Wallace
Oct 18, 2000·The Journal of Biological Chemistry·J LiA Dancis
Oct 12, 2001·Biochemical and Biophysical Research Communications·F LernerM Ziegler
Apr 16, 2002·Molecular Biology of the Cell·María Teresa Rodríguez-ManzanequeEnrique Herrero
May 2, 2002·Nature Genetics·Kam D DahlquistBruce R Conklin
Aug 8, 2002·Human Molecular Genetics·Ulrich MühlenhoffRoland Lill
Sep 11, 2002·Proceedings of the National Academy of Sciences of the United States of America·Opal S ChenJerry Kaplan
Nov 16, 2002·The Journal of Biological Chemistry·Frank PetratHerbert de Groot
May 3, 2003·The EMBO Journal·Caryn E Outten, Valeria C Culotta
Oct 23, 2003·Human Molecular Genetics·Gopalakrishnan KarthikeyanMichael A Resnick
Jan 12, 2005·The Biochemical Journal·Caryn E OuttenValeria C Culotta
Feb 16, 2005·Molecular Genetics and Genomics : MGG·Jean-Guy BerrinMichael Kazmaier
Apr 2, 2005·Nature Reviews. Molecular Cell Biology·Tracey A Rouault, Wing-Hang Tong

❮ Previous
Next ❯

Citations

Oct 12, 2012·Applied Microbiology and Biotechnology·Won-Heong LeeJin-Ho Seo
Nov 18, 2010·The Protein Journal·Baolei JiaGang-Won Cheong
Dec 6, 2012·Nature Communications·Kazuto OhashiKousaku Murata
Jan 23, 2009·The Journal of Biological Chemistry·Hikaru MiyagiKousaku Murata
Jun 27, 2014·PloS One·Wen-Yan LiKun-Ming Chen
May 19, 2009·Metabolic Engineering·Jin HouGoutham N Vemuri
Oct 9, 2008·Metabolic Engineering·Gianni PanagiotouLisbeth Olsson
Feb 14, 2007·The Biochemical Journal·Nadine PollakMathias Ziegler
Dec 1, 2015·Bioorganicheskaia khimiia·D S BilanV V Belousov
Sep 15, 2007·The Journal of Biological Chemistry·Nadine PollakMathias Ziegler
Aug 29, 2021·Molecular Nutrition & Food Research·Jan HomolakMelita Salkovic-Petrisic

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ataxias

Ataxia is a neurological condition characterized by lack of voluntary coordination of muscle movements including loss of coordination, balance, and speech. Discover the latest research on different types of ataxias here.