Genomic characterization of three novel Basilisk-like phages infecting Bacillus anthracis

BMC Genomics
J FarlowM Kutateladze

Abstract

In the present study, we sequenced the complete genomes of three novel bacteriophages v_B-Bak1, v_B-Bak6, v_B-Bak10 previously isolated from historical anthrax burial sites in the South Caucasus country of Georgia. We report here major trends in the molecular evolution of these phages, which we designate as "Basilisk-Like-Phages" (BLPs), and illustrate patterns in their evolution, genomic plasticity and core genome architecture. Comparative whole genome sequence analysis revealed a close evolutionary relationship between our phages and two unclassified Bacillus cereus group phages, phage Basilisk, a broad host range phage (Grose JH et al., J Vir. 2014;88(20):11846-11860) and phage PBC4, a highly host-restricted phage and close relative of Basilisk (Na H. et al. FEMS Microbiol. letters. 2016;363(12)). Genome comparisons of phages v_B-Bak1, v_B-Bak6, and v_B-Bak10 revealed significant similarity in sequence, gene content, and synteny with both Basilisk and PBC4. Transmission electron microscopy (TEM) confirmed the three phages belong to the Siphoviridae family. In contrast to the broad host range of phage Basilisk and the single-strain specificity of PBC4, our three phages displayed host specificity for Bacillus anthracis. Bacill...Continue Reading

References

Nov 1, 1973·Biochemical and Biophysical Research Communications·J A Sands
Dec 1, 1984·Cell·I Katsura, R W Hendrix
Jan 1, 1995·Methods in Enzymology·N D Rawlings, A J Barrett
Oct 6, 2000·Annual Review of Microbiology·I N WangR Young
Jun 5, 2002·Trends in Genetics : TIG·Eduardo P C Rocha, Antoine Danchin
Jul 11, 2002·Journal of Molecular Evolution·Eduard TorrentsFrancisco Rodríguez-Trelles
Apr 23, 2003·Cell·Marisa L PedullaGraham F Hatfull
Jun 12, 2003·Research in Microbiology·Roger W HendrixMargaret C M Smith
Dec 18, 2003·Journal of Bacteriology·Hongbin LiuPhilip C Hanna
Feb 20, 2004·Virology·Mary D RobertsAndrew M Kropinski
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Juan C SalazarDieter Söll
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Harald BrüssowWolf-Dietrich Hardt
Jul 16, 2005·Current Opinion in Microbiology·Sherwood R Casjens
Apr 25, 2006·Journal of Molecular Biology·James S FraserAlan R Davidson
Jun 23, 2006·PLoS Genetics·Graham F HatfullRoger W Hendrix
Aug 28, 2007·Current Opinion in Microbiology·Elizabeth J SummerRy Young
Sep 6, 2007·Genome Research·Marc Bailly-BechetEduardo Rocha
Apr 18, 2008·Briefings in Bioinformatics·Sudhir KumarKoichiro Tamura
Oct 1, 2008·Current Opinion in Microbiology·Graham F Hatfull
Oct 28, 2008·Applied and Environmental Microbiology·Pilar GarcíaJuan E Suárez
Apr 1, 2010·BMC Genomics·Andrew R TimmsIan F Connerton
Sep 20, 2011·Applied and Environmental Microbiology·Dawn B GoldsmithMya Breitbart
Jun 26, 2012·Applied and Environmental Microbiology·Lorena Rodríguez-RubioPilar García
Sep 27, 2012·The Journal of Biological Chemistry·Tomas N GustafssonArne Holmgren
Feb 9, 2013·BMC Evolutionary Biology·Bhakti DwivediMya Breitbart
Jun 20, 2013·BMC Genomics·Kyle C SmithSandra H Burnett
Jul 17, 2013·Biochimica Et Biophysica Acta·Bhaskara L Reddy, Milton H Saier

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Datasets Mentioned

BETA
D4276

Methods Mentioned

BETA
Transmission Electron Microscopy
restriction modification
transglycosylase
aminoacylation

Software Mentioned

BLASTP
ExPASy TMHMM
tRNAscan
PFAM
Geneious
SE
PyMOl
RAST Annotation Pipeline
MEGA

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